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Exome sequencing
Exome sequencing allows targeting the genome coding regions. GenoScreen offers Whole Exome Sequencing (WES) services through the NimbleGen SeqCap EZ System (Roche).
Sanger Sequencing
GenoScreen's historical activity since 2001, Sanger sequencing is used in all fields and for a wide range of applications. It is the standard technique for sequencing DNA accurately and robustly. On our dedicated platform, we offer routine sequencing and, in addition, customised services with various levels of intervention and analysis.
Our Sanger sequencing platform
Equipped with ABI 3730XL capillary sequencers, our platform meets your one-off and regular sequencing needs from tubes, strips or 96-well plates containing your PCR products or vectors.
Molecular barcoding
Molecular barcoding is a technique for identifying and genetically characterising a sample by targeting a ubiquitous gene, often mitochondrial or chloroplast.
Applications:
- Identification of a species from an isolated sample (microorganism, animal or plant species)
- Comparison of several samples from the same species or closely related species
Services offered by GenoScreen
- DNA extraction from any type of matrix (optional)
- PCR amplification, sequencing and assignment of samples to databases (NCBI, BOLD, etc.)
- Construction of phylogenetic trees
- MLST typing and whole genome sequencing (WGS) to characterise the genome
- Solutions for complex matrices via metabarcoding
Discover our services in molecular barcoding
Additional Sanger services
Additional Sanger services
Primer walking
This sequencing method is ideal for:
Principle
The use of several primers makes it possible to obtain overlapping sequences leading to the reconstitution of the complete product. A universal primer is used to sequence an insert, obtaining a fragment of the vector sequence and the beginning of the insert.
A specific primer can then be designed on the sequence obtained and used to obtain the rest of the sequence. If the sequence of interest is known, all the sequencing reactions can be carried out simultaneously. This service can be performed either in single or double reading.
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Mutation detection
Sanger sequencing can be adopted to verify
Principle
Among other applications, the mutation detection is used to monitor TILLING (Targeting Induced Local Lesions in Genomes) in plants, or to verify mutations in genes of interest.
We can design specific primers to target a mutation and compare it to a reference sequence provided for verification.
If several mutations/positions are to be verified in the same genome region, we can also produce a detailed variance report compared with a reference gene.
Identification - Molecular barcoding
Molecular barcoding (DNA barcoding) is a molecular taxonomy technique used to identify and genetically characterise a sample from a ubiquitous gene, generally belonging to the ribosomal, mitochondrial or chloroplast genome (COI, 16S, rbcL, etc.).
DNA barcoding applications
DNA barcoding can be used to:
- Identify species from an isolated sample
- Compare several samples from the same species or closely related species (phylogeny)
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Identification by DNA barcoding
GenoScreen offers a complete molecular barcoding service and can handle your samples from DNA extraction onwards.
- Identification of isolated micro-organisms
- Identification of animal or plant species
For complex inital matrices (e.g. assemblies of bacterial populations), GenoScreen develops metabarcoding solutions using NGS amplicon sequencing to obtain screenings of samples: soil fauna, intestinal flora, coral reefs, etc.
GenoScreen offers you a straight-forward service for identifying your micro-organisms by DNA sequencing and comparison with international databases (NCBI or other).
For species identification, the ribosomal DNA regions are adapted to your micro-organism. In addition, other genomic targets (rpoB, gyrB, panC, etc.) can be used to refine this identification and adapt it to your requirements (for example, discrimination within a group of species sharing the same ribosomal DNA sequence).
Identification services
bacteria
fungi
yeast
microalgae
Workflow for isolated microorganisms
Extraction of your samples
from isolated colonies
PCR amplification on the appropriate genomic target
16S, 18S, 28S, ITS, ...
Forward/reverse
sequencing
Cleaning and assembly
in consensus, then comparison
Assignment
Our offer
Performed on :
- Agar (Petri dishes)
- Liquid culture plate
- FTA card or other matrix
From extracted DNA or thermolysate. We will compare your cleaned and assembled sequences with the international NCBI database.
Once the assignment has been completed, you will receive :
- Raw sequences and chromatograms (.seq/.ab1)
- Sequence consesus (.txt/.FA)
- Analysis report with homology percentages and assignment per sample
Additional solutions
In addition to identification, we are also able to produce phylogenetic trees according to your needs (comparison of samples with each other or within a panel of reference sequences).
Depending on your needs, we can also offer you other targets that are more specific to a particular genus, particularly for species that are too close genetically to the default target (for example, the panC target for the Bacillus genus).
GenoScreen also offers :
for strains whose species is known and for which a typing scheme is available
16S, 18S, 28S, ITS, ...
for more precise characterisation of your micro-organisms
Sequencing
GenoScreen is a major international player providing sequencing and genomic analysis services.
The company’s expertise is based on an unique experience in genomic analyses, the use of cutting-edge technologies and the development and supply of exclusive bioinformatics tools and R&D services. GenoScreen offers a very broad range of sequencing services, from the most basic to the most advanced.
NGS - High-power genomics for the life sciences
Since 2008, GenoScreen offers a full range of next-generation sequencing (NGS) services in application to genomes, metagenomes and transcriptomes.
GenoScreen has cutting-edge technical facilities, giving you access to the latest technologies (Illumina, PacBio, etc.).
The GenoScreen's assets
- A full range of NGS services
- Expert analysis and advice on the design of each analysis
- Unique expertise in microbiology
- High-quality data
- Exclusive bioinformatics tools: assembly, annotation, SNP detection, etc.
- Guaranteed short delay time
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Sanger sequencing - The low-throughput genomics solution
Sanger sequencing is a well-proven, low-throughput analytical solution that is well suited to fragments of all sizes (100-1200 bp read length). GenoScreen’s Sanger sequencing services can be adapted to suit all types of project, regardless the size, delay time or frequency.
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Sanger sequencing applications
Despite the emergence of new generation technologies (NGS), Sanger sequencing remains a reference method in many fields thanks to its reliability and accuracy.
Flexible and available, our teams carry out sequencing from sample preparation to data analysis. See our documentation below to find out more about our services and to prepare your sequencing request form.
User manual
Form (with purification)
Form (without purification)
Premium range
The Premium offers correspond to a complete and personalised analysis of samples:
- Adaptation of the protocol to GC-rich sequences
- Supply of universal primers
- Sequence reading up to 1200 bp
- Results sent within 24 to 48 hours
The Premium range gives you access to our hotline, by phone and email: expert scientific support for sequence profile analysis.
Workflow tailored to your needs
Purification and dosing
Sequencing reactions
Purification and migration on capillary sequencer
Deliverables with different levels of analysis
After-sales service, eventual reprocessing with optimised protocol
One-Shot
Free purification
Deliverables
.seq / .ab1
Monthly activity report (including remaining credits)
One-Shot Plus
Deliverables
+
Semi-automated review of sequences and corrections with IUPAC annotations
Optimized
Deliverables
+
Manual IUPAC annotations
Reprocessing with optimised protocol (on request)
All our offers are open to different sample formats:
- tubes
- strips
- plates, complete or not
Our One-Shot Plus and Optimized offers include additional information:
- Signal optimisation
- Reduction of any backgroun noise
- Assignment of indeterminate bases
- A specific protocol for mutation detection
Additional analyses
GenoScreen offers a range of services to complement sequencing:
Economic range
All the quality and responsiveness of GenoScreen at a low price!
Using your own PCR product + primer mix or even the sequencing reaction you have produced, we can take your samples to a more advanced stage for an optimised price.
GenoScreen advantages
- Flexible sequencing offer that adapts to your needs
-
Personalised monitoring of your projects
-
Simple ordering system
-
Monthly invoicing or prepaid account
-
Free transport of your samples by a GenoScreen-approved operator
-
Optimised protection of samples in Genobox
Subcategories
GenoScreen
Collaborative research - At the heart of international innovation in genomics
GenoScreen is a partner in collaborative research projects that bring together startups, multinationals and public-sector organizations. Our R&D teams provide their knowledge and expertise in the molecular microbiology of isolated agents and complex communities. This allowed our teams to develop our own projects for elaborating innovative products and services.
Our projects are designed to:
- Improve the diagnosis and management of acute/chronic diseases on humans and animals,
- Characterize and monitor microbial biodiversity, with applications in agronomy, agrifood and environment.
The common feature of these projects is the development of molecular tools for the characterization, monitoring and diagnosis of microbial communities. The key objective is to market simple analytical solutions and (ultimately) preventive, corrective or even therapeutic products based on microorganisms.