Sanger sequencing - The low-throughput genomics solution

Sanger sequencing is a well-proven, low-throughput analytical solution that is well suited to fragments of all sizes (100-1200 bp read length). GenoScreen’s Sanger sequencing services can be adapted to suit all types of project, regardless the size, delay time or frequency.

This email address is being protected from spambots. You need JavaScript enabled to view it.

Sanger sequencing applications

Despite the emergence of new generation technologies (NGS), Sanger sequencing remains a reference method in many fields thanks to its reliability and accuracy.

GenoScreen offer

Flexible and available, our teams carry out sequencing from sample preparation to data analysis. See our documentation below to find out more about our services and to prepare your sequencing request form.

User manual

 

Form (with purification)

Form (without purification)

 

Premium range

The Premium offers correspond to a complete and personalised analysis of samples:

• Adaptation of the protocol to GC-rich sequences
• Supply of universal primers
• Sequence reading up to 1200 bp
• Results sent within 24 to 48 hours

The Premium range gives you access to our hotline, by phone and email: expert scientific support for sequence profile analysis.

Workflow tailored to your needs

Features

Purification and dosing

Sequencing reactions

Purification and migration on capillary sequencer

Deliverables with different levels of analysis

After-sales service, eventual reprocessing with optimised protocol

Pricing

One-Shot

Ask for a quote

Free purification

Deliverables

.seq / .ab1

Monthly activity report (including remaining credits)

One-Shot Plus

Ask for a quote

Deliverables

One-Shot
+

Semi-automated review of sequences and corrections with IUPAC annotations

Optimized

Ask for a quote

Deliverables

One-Shot Plus
+

Manual IUPAC annotations

Reprocessing with optimised protocol (on request)

One-Shot

Demander un devis

Purification gratuite

Livrables

.seq / .ab1

Rapport mensuel d'activité (incluant les crédits restants)

One-Shot Plus

Demander un devis

Livrables

One-Shot
+

Relecture des séquences et corrections avec annotations IUPAC semi-automatisée

Optimized

Demander un devis

Livrables

One-Shot Plus
+

Annotations IUPAC manuelle

Rapport QC (Quality control) relecture manuelle et commentée de chaque séquence avec score qualité

Retraitement avec protocole optimisé (sur demande)

All our offers are open to different sample formats:

• tubes
• strips
• plates, complete or not

Our One-Shot Plus and Optimized offers include additional information:

• Signal optimisation
• Reduction of any backgroun noise
• Assignment of indeterminate bases
• A specific protocol for mutation detection

Additional analyses

GenoScreen offers a range of services to complement sequencing:

Ask for a quote

Economic range

All the quality and responsiveness of GenoScreen at a low price!

Using your own PCR product + primer mix or even the sequencing reaction you have produced, we can take your samples to a more advanced stage for an optimised price.

GenoScreen advantages

• Flexible sequencing offer that adapts to your needs

• Personalised monitoring of your projects

• Simple ordering system

• Monthly invoicing or prepaid account

• Free transport of your samples by a GenoScreen-approved operator

• Optimised protection of samples in Genobox

Metagenomics - Powerful analyses of microbial communities

At GenoScreen, we turn metagenomic data into actionable insights through tailored analyses and cutting-edge scientific support.

This email address is being protected from spambots. You need JavaScript enabled to view it.

Why choose metagenomics?

Metagenomics allows the analysis of the diversity and functioning of microbial communities directly in their environment, without prior culturing. It offers a deep understanding of the composition, interactions, and functions of the microbiome, paving the way for major applications in human and animal health, dermocosmetics, environmental studies, and industrial biotechnologies.

Slide 1

20+ years of experience

in genomics &
metagenomics

Metagenomics: Our Expertise

Our experience has allowed us to develop and refine robust methodologies tailored to the uniqueness of each sample type, ensuring the relevance and reliability of your results

You will be supported by a multidisciplinary team of experts in bioinformatics and microbiology, ensuring comprehensive and personalized project support.

Quality assurance, the core of our approach

We have implemented a systematic and thorough internal quality control system to validate the performance of our protocols and ensure the integrity of your results.

You will benefit from clear and interactive results, designed for in-depth understanding and optimal use of your data.

GCLP-compliant

rigorous &
traceable analyses

Methodologies adapted to every sample type

Each biological or environmental matrix is unique and requires a specific approach. Through our expertise, we have developed optimized protocols for various matrices:

Gut microbiota

Skin microbiota

Soils microbiota

Others

Other sample types? Our experience allows us to adapt our methods to a wide variety of samples.
Let’s talk about it!

Contact us

Our workflow applied to metagenomics

Optimized DNA extraction for each matrix

stool, soil, skin, fermented samples, etc.

Library preparation

with integrated quality controls

High-throughput sequencing adapted to your needs

Bioinformatics analysis

Interpretation & reporting

More technologies, more personalisation

We offer DNA sequencing technologies fully tailored to your analytical goals : Illumina (short-read) for high-throughput, accurate sequencing, and PacBio (long-read) for high-resolution insights, ideal for detailed microbial genome characterization.

Depending on the level of analysis required, our protocols are available in metabarcoding (16S, 18S, ITS) for a targeted approach, or shotgun metagenomics for a comprehensive exploration of microbial diversity, both taxonomic and functional.

Additionally, sequencing depth is adjusted to your specific needs, taking into account the richness and complexity of the microbiota under study(whether human, animal, plant, or environmental in origin).

Pricing

The GenoScreen experience in a nutshell...

From setting up your project to delivering the results

Methods and solutions tailored to your projects

Micro-organism studies for over 20 years

Support beyond results delivery

‘materials and methods’ explained

Targeted sequencing - An in-depth analysis of regions of interest

Targeted sequencing allows specific analysis of targeted genes (or gene regions) on many samples in parallel. With NGS sequencing on MiSeq^®, NextSeq500^® or Hiseq^® platforms, multiple genes can be evaluated with a smaller data volume, which is easier to manage and analyse than WGS datas.

Applications

Exome sequencing

This type of sequencing is essentially used to:

• Screen for mutations in genes involved in regulatory pathways
• Screen for mutations (SNPs, indels, etc.) associated with a particular disease (cancer, coagulopathies, etc.).

We use the NimbleGen SeqCap EZ protocol (Roche) for exome sequencing.

Personalized sequencing

After custom probe design, your regions of interest are enriched prior to sequencing which simplifies the subsequent data analysis. This protocol can be set up for any organism with an available reference genome.

GenoScreen's assets

• A dedicated team of scientists for each project.
• Comprehensive support with the elaboration of an experimental plan and project implementation.
• Assistance with data handling and interpretation of the results.

Ask for a quotation

Ready-to-load services - Plugged into our sequencing platform

For a cost effective and responsive offer, GenoScreen proposes "Ready to Load" sequencing services for a wide range of libraries: a real research accelerator giving the opportunity to perform on last generation technical platform with advanced expertise.

Applications

Transcriptomics - Analyzing gene expression

High-throughput sequencing of the transcriptome (RNA-Seq) has revolutionized the quantitative and qualitative analysis of prokaryotic and eukaryotic organisms.

Whole-genome sequencing - A high-precision method for characterizing the genome

The latest sequencing technologies make it possible to analyze the full primary structure of an isolated organism’s genome, regardless of, whether the latter is a eukaryote, a prokaryote or a virus.
An analysis of whole-genome sequencing data provides a precised description of any type of organism and thus addresses a very broad range of research questions.

Applications

MLST/MLVA, microbial typing/tracing solutions

GenoScreen proposes you the opportunity to characterize more accurately your microorganisms (subspecies or strain characterization) using standardized and validated molecular biology methods such as MLST or MLVA (including MIRU-VNTR).

MultiLocus Sequencing Typing (MLST)

Pricing

The MLST (MultiLocus Sequencing Typing) sequencing typing is the reference technique for the distinction of different strains. This method is based on the house-keeping genes sequencing (house-keeping genes are encoding essential proteins of the bacterium).

These sequences have the particularity of presenting a stable polymorphism over time and sufficient enough to distinguish strains from each other.

This approach allows the characterization of a microorganism whose genus (or species) is known in order to identity the species(or subspecies) and this thanks to the sequencing of 7 house-keeping genes.

Ask for a quote

The MLST by GenoScreen

GenoScreen's MLST service offer is a complete package of services realized from extracted DNA or thermolysate. This includes the PCRs realization, the sequencing on a capillary sequencer, the sequences analysis and the strain assignment..

Multiple Loci VNTR Analysis (MLVA)

Pricing

The MLVA (Multiple Loci VNTR Analysis) is a genetic analysis method of VNTR (variable number of tandem repeats) pattern polymorphism based on their count. During a first step, each targeted VNTR locus is amplified by PCR with flanking-region specific primers. The fragments obtained are then separated according to their size by electrophoresis on capillary sequencer. The genotyping of each locus and the results compiled from each sample allow to assign to the strain a specific numerical code for a subspecies.

The MLVA approach allows to characterize a microorganism whose species is known, to identify, at least, the subspecies through allele typing and comparison to the MLVA databases. This method is more selective than the MLST method and does not require a DNA sequencing step. It thus makes it possible to differentiate close subspecies (clonal species).

Ask for a quote

Le MLVA par GenoScreen

GenoScreen's MLVA service offer is a complete package of services realized from extracted DNA or thermolysate. This includes the PCRs realization, the electrophoresis on a capillary sequencer, the data analysis and the strain assignment. 

The analysis can be carried out on the basis of a personalised typing scheme tailored to your needs, in line with the data available for your species.