GenoScreen services
NGS - High-power genomics for the life sciences
Since 2008, GenoScreen offers a full range of next-generation sequencing (NGS) services in application to genomes, metagenomes and transcriptomes.
GenoScreen has cutting-edge technical facilities, giving you access to the latest technologies (Illumina, PacBio, etc.).
The GenoScreen's assets
- A full range of NGS services
- Expert analysis and advice on the design of each analysis
- Unique expertise in microbiology
- High-quality data
- Exclusive bioinformatics tools: assembly, annotation, SNP detection, etc.
- Guaranteed short delay time
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Sanger sequencing - The low-throughput genomics solution
Sanger sequencing is a well-proven, low-throughput analytical solution that is well suited to fragments of all sizes (100-1200 bp read length). GenoScreen’s Sanger sequencing services can be adapted to suit all types of project, regardless the size, delay time or frequency.
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Sanger sequencing applications
Despite the emergence of new generation technologies (NGS), Sanger sequencing remains a reference method in many fields thanks to its reliability and accuracy.
Flexible and available, our teams carry out sequencing from sample preparation to data analysis. See our documentation below to find out more about our services and to prepare your sequencing request form.
User manual
Form (with purification)
Form (without purification)
Premium range
The Premium offers correspond to a complete and personalised analysis of samples:
- Adaptation of the protocol to GC-rich sequences
- Supply of universal primers
- Sequence reading up to 1200 bp
- Results sent within 24 to 48 hours
The Premium range gives you access to our hotline, by phone and email: expert scientific support for sequence profile analysis.
Workflow tailored to your needs
Purification and dosing
Sequencing reactions
Purification and migration on capillary sequencer
Deliverables with different levels of analysis
After-sales service, eventual reprocessing with optimised protocol
One-Shot
Free purification
Deliverables
.seq / .ab1
Monthly activity report (including remaining credits)
One-Shot Plus
Deliverables
+
Semi-automated review of sequences and corrections with IUPAC annotations
Optimized
Deliverables
+
Manual IUPAC annotations
Reprocessing with optimised protocol (on request)
All our offers are open to different sample formats:
- tubes
- strips
- plates, complete or not
Our One-Shot Plus and Optimized offers include additional information:
- Signal optimisation
- Reduction of any backgroun noise
- Assignment of indeterminate bases
- A specific protocol for mutation detection
Additional analyses
GenoScreen offers a range of services to complement sequencing:
Economic range
All the quality and responsiveness of GenoScreen at a low price!
Using your own PCR product + primer mix or even the sequencing reaction you have produced, we can take your samples to a more advanced stage for an optimised price.
GenoScreen advantages
- Flexible sequencing offer that adapts to your needs
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Personalised monitoring of your projects
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Simple ordering system
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Monthly invoicing or prepaid account
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Free transport of your samples by a GenoScreen-approved operator
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Optimised protection of samples in Genobox
Metagenomics - Powerful analyses of microbial communities
At GenoScreen, we turn metagenomic data into actionable insights through tailored analyses and cutting-edge scientific support.
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Why choose metagenomics?
Metagenomics allows the analysis of the diversity and functioning of microbial communities directly in their environment, without prior culturing. It offers a deep understanding of the composition, interactions, and functions of the microbiome, paving the way for major applications in human and animal health, dermocosmetics, environmental studies, and industrial biotechnologies.
in genomics &
metagenomics
Metagenomics: Our Expertise
Our experience has allowed us to develop and refine robust methodologies tailored to the uniqueness of each sample type, ensuring the relevance and reliability of your results
You will be supported by a multidisciplinary team of experts in bioinformatics and microbiology, ensuring comprehensive and personalized project support.
Quality assurance, the core of our approach
We have implemented a systematic and thorough internal quality control system to validate the performance of our protocols and ensure the integrity of your results.
You will benefit from clear and interactive results, designed for in-depth understanding and optimal use of your data.
rigorous &
traceable analyses
Methodologies adapted to every sample type
Each biological or environmental matrix is unique and requires a specific approach. Through our expertise, we have developed optimized protocols for various matrices:
Other sample types? Our experience allows us to adapt our methods to a wide variety of samples.
Let’s talk about it!
Our workflow applied to metagenomics
Optimized DNA extraction for each matrix
stool, soil, skin, fermented samples, etc.
Library preparation
with integrated quality controls
High-throughput sequencing adapted to your needs
Bioinformatics analysis
Interpretation & reporting
More technologies, more personalisation
We offer DNA sequencing technologies fully tailored to your analytical goals : Illumina (short-read) for high-throughput, accurate sequencing, and PacBio (long-read) for high-resolution insights, ideal for detailed microbial genome characterization.
Depending on the level of analysis required, our protocols are available in metabarcoding (16S, 18S, ITS) for a targeted approach, or shotgun metagenomics for a comprehensive exploration of microbial diversity, both taxonomic and functional.
Additionally, sequencing depth is adjusted to your specific needs, taking into account the richness and complexity of the microbiota under study(whether human, animal, plant, or environmental in origin).
The GenoScreen experience in a nutshell...
From setting up your project to delivering the results
Methods and solutions tailored to your projects
Micro-organism studies for over 20 years
Support beyond results delivery
‘materials and methods’ explained
Targeted sequencing - An in-depth analysis of regions of interest
Targeted sequencing allows specific analysis of targeted genes (or gene regions) on many samples in parallel. With NGS sequencing on MiSeq®, NextSeq500® or Hiseq® platforms, multiple genes can be evaluated with a smaller data volume, which is easier to manage and analyse than WGS datas.
Applications
Sequencing or targeted resequencing can achieve very specific objectives:
- Screening of genomic regions of interest.
- Complete coverage of coding regions, with a limited volume of genomic data.
- Analysis of microbial genes.
GenoScreen - Services provided
Amplicon sequencing
A number of the target regions are amplified by PCR, using the initially defined specific primers. The amplicons obtained are then sequenced by NGS, following the addition of sequencing adaptors. Multiplexing approaches enable us to sequence several amplicons from different samples in parallel.
The Metabiote® solution
Metabiote® is a dedicated, innovative, optimized, standardized solution developed by GenoScreen. It integrates targeted metagenomic analysis of microbial communities.
- A library preparation methodology that limits between-sample bias.
- A diverse range of targets:
• bacterial targets: 16S rDNA
• fungal targets: 18S, ITS1 and/or ITS2 rDNA - A bioinformatics pipeline that has been optimized and automated for high-precision analyses, easy comparisons and statistical analyses, and a high degree of responsiveness.
Exome sequencing
This type of sequencing is essentially used to:
- Screen for mutations in genes involved in regulatory pathways
- Screen for mutations (SNPs, indels, etc.) associated with a particular disease (cancer, coagulopathies, etc.).
We use the NimbleGen SeqCap EZ protocol (Roche) for exome sequencing.
Personalized sequencing
After custom probe design, your regions of interest are enriched prior to sequencing which simplifies the subsequent data analysis. This protocol can be set up for any organism with an available reference genome.
GenoScreen's assets
- A dedicated team of scientists for each project.
- Comprehensive support with the elaboration of an experimental plan and project implementation.
- Assistance with data handling and interpretation of the results.
Ready-to-load services - Plugged into our sequencing platform
For a cost effective and responsive offer, GenoScreen proposes "Ready to Load" sequencing services for a wide range of libraries: a real research accelerator giving the opportunity to perform on last generation technical platform with advanced expertise.
Applications
"Ready-to-load" applications are available for mainly all of GenoScreen’s sequencing services:
- De novo sequencing or re-sequencing.
- RNA-Seq.
- Targeted sequencing.
- Metagenomics.
GenoScreen - Services provided
"Ready-to-load" sequencing is performed on latest-generation technical facilities: Illumina HiSeq®2500, MiSeq®, NextSeq®500 and HiSeq®4000 systems in paired-end or single-end runs of 50 to 250 bp.
GenoScreen performs quality control on the libraries with bioAnalyzer and fluorimetric assays. GenoScreen can also ensure the pooling of the sequencing libraries, if required.
We accept different types of librairies:
- TruSeq.
- Nextera(-XT).
- Amplicons.
- RADseq or ddRADseq with index and Illumina adaptors.
For the other types of librairies, please contact us.
The results
- Raw sequences (FastQ files) and quality control results after demultiplexing.
- Quality reports, including the number of reads obtained, the number of reads after application of quality filters, and the coverage obtained.
- The results are transferred to a secure FTP server or an external hard disk.
GenoScreen's assets
- Cutting-edge technical facilities.
- A top-of-the-range service at a keen price.
- Responsiveness.
Transcriptomics - Analyzing gene expression
What is transcriptomics and why study it?
Transcriptomics determines which genes are actively expressed, to what extent under specific conditions. With high-throughput sequencing technologies, it provides a comprehensive and quantitative view of gene activity in both prokaryotic and eukaryotic organisms.
Today, transcriptomics is one of the most powerful methods to understand cellular responses to stress, treatments, environmental changes, or developmental stages.
Our RNA-Seq applications
Our services cover all transcriptomic analysis needs:
- Differential expression analysis between conditions or treatments
- Transcript abundance quantification (mRNA)
- Functional gene annotation
- Detection of single nucleotide variants (SNPs) from transcripts
- Multi-omics integration with genomic or metagenomic data
Want to focus on a few genes of interest? We also offer targeted quantification via RT-qPCR, a fast, economical solution.
RNA-Seq Workflow: from sample to results
Experimental design
RNA Extraction
Optimized protocols
Sequencing
Differential expression analysis
Comprehensive Reports (Physical & Summary)
Dedicated scientific team for each project.
Assistance in developing the experimental plan and sequencing strategy
Two methods are used to enrich the RNA fraction of interest (mRNA) prior to cDNA synthesis and library preparation:
- From total eukaryotic RNA: Capture of mRNAs by their polyA tail
- From total prokaryotic or eukaryotic RNA: Depletion of rRNA through capture by complementary probes. Enrichment of samples in mRNA
Comprehensive processing of sequencing data
Differential expression analysis
Functional enrichment analysis
Interactive reports for intuitive exploration of your data:
- Dynamic visualisations (graphs, tables)
- Filtering and sorting tools to target genes or metabolic pathways of interest
Interpretation of results and personalised support for independent use
Whole-genome sequencing - A high-precision method for characterizing the genome
The latest sequencing technologies make it possible to analyze the full primary structure of an isolated organism’s genome, regardless of, whether the latter is a eukaryote, a prokaryote or a virus.
An analysis of whole-genome sequencing data provides a precised description of any type of organism and thus addresses a very broad range of research questions.
Applications
Whole-genome sequencing addresses important research questions in many sectors (healthcare, nutrition, agrifood, environment, etc.):
- Description of an organism’s genes and how they are organized.
- Quantification of diagnostic or phenotypic biomarkers.
- Screening for susceptibility/adaptability/resistance genes.
GenoScreen - Services provided
As specialists in whole-genome sequencing (WGS), our expert teams are able to build you a custom solution.
Our precise, fast solutions combine NGS and Sanger techniques and are implemented in cutting-edge technical facilities.
Our bioinformatics solutions can, then deliver in-depth processing and analysis of your WGS data (assembly, annotation, etc.).
- Genome structure and mapping of isolated organisms.
- Comparative genomics: identification of SNPs, indels and/or major structural rearrangements.
- Multilocus sequence typing (MLST).
GenoScreen's assets
- We understand your needs and provide personalized support.
- Exclusive bioinformatics analyses.
- Significant expertise in sequencing microorganisms (bacteria, fungi, yeasts, algae, etc.).
- Fast solution.