SSR microsatellite genotyping

This technique is based on the amplification of short DNA sequences composed of repeating units of a few nucleotides. Because of their polymorphism, microsatellite markers can be used to study genetic diversity within a species or population. GenoScreen has developed almost 20 years of expertise in the study and analysis of microsatellites, enabling the development of markers for more than 500 species.

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When should microsatellite genotyping be used?

GenoScreen offer

 

 

 

Use of existing data

As microsatellite markers are molecular reference tools, there are many publications and previous works available today that we can draw on to meet your needs. We can also use existing data on closely related species (cross-amplification) or from high-throughput sequencing.

Whenever markers are already available for your species of interest, we can use the amplification conditions developed or develop new ones to, for example, group together markers from different publications or use only some of them. There are several possible scenarios:

Features

Multiplex development

(several pairs of primers)

From previously published publications / markers

Cross-amplification

Custom

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Development of de novo markers

 GenoSat® - GenoScreen offer

Geno Sat^® is a standardised, simple and modular solution for all types of eukaryotic species. Since 2009, our solution has already been used on more than 500 different species as part of phylogenetic studies, biodiversity analyses and conservation programmes. This solution enables :

• Production of microsatellite libraries using high-throughput sequencing,
• Development of polymorphic markers of interest
• Population genotyping

Pricing

Geno Sat®

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Using 1 µg of genomic DNA and Illumina MiSeq high-throughput sequencing technology, GenoSat® can produce bioinformatically validated primer pairs within 2 months, framing the microsatellites identified for your species.

Geno Sat® Pack

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This modular solution lets you both biologically validate a batch of primer pairs (after in silico validation), and study the polymorphism of successfully amplified pairs.

Geno Sat® Pack +

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The Geno Sat® Pack + solution is our most comprehensive offering for optimising multiplexes and/or genotyping large populations using polymorphic microsatellite markers of interest.

Discover the list of species analysed with Geno Sat® below:

 

Fragment analysis

Equipped with ABI 3730XL capillary sequencers, we can respond rapidly to your needs for fragment analysis (microsatellites, SSR, STR, etc.) using your labelled PCR products supplied in 96-well plates.

We offer sliding scale prices depending on the number of plates received per batch and migrated with the same size marker.

It is also possible to send us plates ready to be migrated using the same principle, already containing the Hi-Di Formamide + size marker mix with your sample.

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