Sanger sequencing is a well-proven, low-throughput analytical solution that is well suited to fragments of all sizes (100-1200 bp read length). GenoScreen’s Sanger sequencing services can be adapted to suit all types of project, regardless the size, delay time or frequency.

 

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Sanger sequencing applications

 

Despite the emergence of new generation technologies (NGS), Sanger sequencing remains a reference method in many fields thanks to its reliability and accuracy.

 

 

Flexible and available, our teams carry out sequencing from sample preparation to data analysis. See our documentation below to find out more about our services and to prepare your sequencing request form.

 

User manual

download our user manual 

Form (with purification)

séquençage avec purification

Form (without purification)

séquençage sans purification 

 

  

Premium range

The Premium offers correspond to a complete and personalised analysis of samples:résultats Sanger sous 24 à 48h

  • Adaptation of the protocol to GC-rich sequences
  • Supply of universal primers
  • Sequence reading up to 1200 bp
  • Results sent within 24 to 48 hours

 

The Premium range gives you access to our hotline, by phone and email: expert scientific support for sequence profile analysis.

 

Workflow tailored to your needs

Sanger sequencing - The low-throughput genomics solution Workflow Sanger
Purification and dosing

Purification and dosing

Sequencing reactions

Sequencing reactions

Purification and migration on capillary sequencer

Purification and migration on capillary sequencer

Deliverables with different levels of analysis

Deliverables with different levels of analysis

After-sales service, eventual reprocessing with optimised protocol

After-sales service, eventual reprocessing with optimised protocol

Sanger Premium
Purification and dosing

Free purification

Deliverables

Deliverables with different levels of analysis

.seq / .ab1

Monthly activity report (including remaining credits)

One-Shot Plus

Deliverables

One-Shot
+
Purification and migration on capillary sequencer

Semi-automated review of sequences and corrections with IUPAC annotations

Optimized

Deliverables

One-Shot Plus
+
Purification and migration on capillary sequencer

Manual IUPAC annotations

After-sales service, eventual reprocessing with optimised protocol

Reprocessing with optimised protocol (on request)

 

All our offers are open to different sample formats:

  • tubes
  • strips
  • plates, complete or not

 

Our One-Shot Plus and Optimized offers include additional information:

  • Signal optimisation
  • Reduction of any backgroun noise
  • Assignment of indeterminate bases
  • A specific protocol for mutation detection

 

Additional analyses

 

GenoScreen offers a range of services to complement sequencing:

Sanger sequencing - The low-throughput genomics solution Additional analyses
Purification from pre-cut agarose strips
Custom primer orders
Primer
walking
Mutation
detection
phylogenetic tree editing
Custom analysis
assembly of
forward
and
reverse
readings
generation
of
consensus
sequences
(F/R assembly)

 

Ask for a quote

 

 

Economic range

All the quality and responsiveness of GenoScreen at a low price!

Using your own PCR product + primer mix or even the sequencing reaction you have produced, we can take your samples to a more advanced stage for an optimised price.

 

 

Sanger Economic

Mix & Run

DNA mix and primers produced on your own (in plates)

Sequencing reaction, purification and migration on capillary sequencer

Sequencing reactions carried out on your own (in plates)

Purification and migration on capillary sequencer

Mélange ADN et primers réalisés par vos soins (en plaque)

Réaction de séquençage, purification et migration sur séquenceur capillaire

Réactions de séquençage réalisées par vos soins (en plaque)

Purification et migration sur séquenceur capillaire

 

GenoScreen advantages

  • Flexible sequencing offer that adapts to your needs
  • Personalised monitoring of your projects

  • Simple ordering system

  • Monthly invoicing or prepaid account

  • Free transport of your samples by a GenoScreen-approved operator

  • Optimised protection of samples in Genobox

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