Genomic information at the service of human health and its environment
Sequencing
GenoScreen is a major international player providing sequencing and genomic analysis services.
The company’s expertise is based on an unique experience in genomic analyses, the use of cutting-edge technologies and the development and supply of exclusive bioinformatics tools and R&D services. GenoScreen offers a very broad range of sequencing services, from the most basic to the most advanced.
Sanger sequencing
GenoScreen Sanger sequencing services can be adapted to any type of project: magnitudes, delays and production frequencies, for a reading between 100 and 1200 bp.
Next-generation sequencing
GenoScreen offers a complete range of Next Generation Sequencing (NGS) services updated with the latest technologies (Illumina, PacBio ...).
Since 2008, GenoScreen offers a full range of next-generation sequencing (NGS) services in application to genomes, metagenomes and transcriptomes.
GenoScreen has cutting-edge technical facilities, giving you access to the latest technologies (Illumina, PacBio, etc.).
GenoScreen - Services provided
- Whole-genome sequencing (WGS)
New sequencing technologies available at GenoScreen offer the opportunity to analyse the entire primary structure of an isolated organism genome, whether eukaryotic, prokaryotic or viral. The analysis of data obtained allows to precisely characterize and describe any type of organisation and thus responds to a large panel of research problems. - Metagenomics
Metagenomics molecular approaches allows to analyse a community of organisms as a whole and to study a complex ecological niche exhaustively: a powerful and innovative approach to characterize the genes and, therefore, the biological functions carried by these microbiomes. - Targeted sequencing
Targeted sequencing allows specific analysis of targeted genes (or gene regions) on many samples in parallel. With NGS sequencing on MiSeq®, NextSeq500® or Hiseq® platforms, multiple genes can be evaluated with a smaller data volume, which is easier to manage and analyse than WGS datas. - Transcriptomics
High-throughput transcriptome sequencing (RNA-seq) has revolutionised prokaryotic and eukaryotic organisms quantitative and qualitative analyses. It allows, in particular, to perform differential expression analyses. - Ready to load
For a cost effective and responsive offer, GenoScreen proposes "Ready to Load" sequencing services for a wide range of libraries: a real research accelerator giving the opportunity to perform on last generation technical platform with advanced expertise.
The GenoScreen's assets
- A full range of NGS services
- Expert analysis and advice on the design of each analysis
- Unique expertise in microbiology
- High-quality data
- Exclusive bioinformatics tools: assembly, annotation, SNP detection, etc.
- Guaranteed short delay time
Sanger sequencing is a well-proven, low-throughput analytical solution that is well suited to fragments of all sizes (100-1200 bp read length).
GenoScreen’s Sanger sequencing services can be adapted to suit all types of project, regardless the size, delay time or frequency.
GenoScreen - Services provided
Our responsive teams perform the sequencing from the preparation of your samples until the analysis of your data
The Premium range
Take full advantage of our expertise!
The Premium range corresponds to the full, personalized analysis of your samples:
- Adaptation of the protocol to GC-rich sequences
- Supply of universal primers
- A read length of up to 1200 bp
- Delay time within 24 hours
The Premium range gives you access to our hotline: expert scientific support for the analysis of sequence profiles.
| One-Shot | One-Shot Plus | Optimized | |
| Results within 24 to 48 hours after receipt | x | x | x |
| Technical support by email and/or phone | x | x | x |
Accepted sample formats:
|
x | x | x |
| Free sample purification (on request) | x | x | x |
| Reading up to 1200 bp | x | x | x |
| Sequence review and corrections with IUPAC annotations | Semi-automated | Manual | |
| Deliverables | .seq / .ab1 | .seq / .ab1 | .seq / .ab1 QC (Quality control) report with comments for each sequence |
| Reprocessing with optimized protocol (on request) | x | ||
| Monthly activity report (including remaining credits) | x | x | x |
The Economy range
Access GenoScreen’s quality and responsiveness at keen prices!
| Mix & Run | Run Only |
|---|---|
| You provide the DNA/primer reaction mixture in microplates | |
| Sequencing (BigDye® Terminator) | You perform the sequencing reactions and supply the microplates |
| Clean-up of the sequencing products for gel migration | Clean-up of the sequencing products for gel migration |
| Migration on an ABI 3730 xl system | Migration on ABI 3730 xl system |
Additional analyses
Further on, GenoScreen offers additional services to complement the sequencing steps:
- Extraction of plasmid DNA from bacterial cultures.
- Extraction of BACs and cosmids.
- Purification of PCR products and gel cut-out.
- Primer synthesis.
- Analysis of the results according to IUPAC codes.
GenoScreen's assets
- Flexible sequencing services that meet your needs.
- Personalized project monitoring.
- An easy-to-use ordering system.
- Monthly invoicing or a prepaid customer account.
- Free sample shipping by a GenoScreen-approved courier.
- Optimized sample protection in a Genobox.
Documentation
Molecular metagenomics is used to analyze a community of organisms as a whole; as such, it provides an exhaustive assessment of a complex ecological niche. It is a powerful, innovative approach for characterizing the genes and thus the biological functions in a given microbiome.
In contrast to targeted (profiling) approaches that examine specific, short regions of ribosomal genes, metagenomics is based on the full sequencing of the DNA extracted from a sample (shotgun sequencing).
Metagenomics requires a huge sequencing coverage per sample. This is a limitation for studies of large cohorts, but it provides valuable additional information when profiling a representative subgroup.
Metagenomics has many advantages:
- Removal of the bias associated with PCR approaches
- Culture-free analysis and direct sequencing of microorganisms
- The functional description of a whole ecosystem
Applications
Metagenomics provides an exhaustive analysis of the taxonomic composition of a community of organisms. It provides precise information on the community’s diversity and specific genetic characteristics.
In credit to this direct genetic analysis of microbial communities, metagenomics can also be used to identify new enzymes (biocatalysts) for pharmaceutical, agricultural, industrial and environmental applications.
Metagenomics and human health
Metagenomics is a very well suited solution in diagnosis, evaluation of antibiotic resistance and virus detection, amongst other topics.
It delivers precised solutions in oncology, gastroenterology, dermatology, urology and pulmonology.
Metagenomics and the environment
In credit to its very detailed analysis of microbial communities present in the natural environment, metagenomics can be applied to the analysis of water quality, the development of biofuels and agrochemical products, soil remediation and the development of new practices in agriculture and animal husbandry.
GenoScreen - Services provided
GenoScreen’s standardized methods for metagenomic analysis are able to address all the different aspects of your project. By combining low-, medium- and/or high-coverage sequencing with library preparation (with or without a PCR step), we can offer the solution that best meets your needs.
GenoScreen guarantees the quality of its data produced and uses its exclusive WHORMSS® software to generate a detailed set of results.
GenoScreen's assets
- A dedicated team of expert scientists for each project.
- Assistance with developing the experimental plan and the sequencing strategy.
- Massive whole metagenome sequencing capacity.
- Exclusive bioinformatics tools.
- Cutting-edge scientific expertise for data exploitation (specially in the field of microbiology).
Targeted sequencing allows specific analysis of targeted genes (or gene regions) on many samples in parallel. With NGS sequencing on MiSeq®, NextSeq500® or Hiseq® platforms, multiple genes can be evaluated with a smaller data volume, which is easier to manage and analyse than WGS datas.
Applications
Sequencing or targeted resequencing can achieve very specific objectives:
- Screening of genomic regions of interest.
- Complete coverage of coding regions, with a limited volume of genomic data.
- Analysis of microbial genes.
GenoScreen - Services provided
Amplicon sequencing
A number of the target regions are amplified by PCR, using the initially defined specific primers. The amplicons obtained are then sequenced by NGS, following the addition of sequencing adaptors. Multiplexing approaches enable us to sequence several amplicons from different samples in parallel.
The Metabiote® solution
Metabiote® is a dedicated, innovative, optimized, standardized solution developed by GenoScreen. It integrates targeted metagenomic analysis of microbial communities.
- A library preparation methodology that limits between-sample bias.
- A diverse range of targets:
• bacterial targets: 16S rDNA
• fungal targets: 18S, ITS1 and/or ITS2 rDNA - A bioinformatics pipeline that has been optimized and automated for high-precision analyses, easy comparisons and statistical analyses, and a high degree of responsiveness.
Exome sequencing
This type of sequencing is essentially used to:
- Screen for mutations in genes involved in regulatory pathways
- Screen for mutations (SNPs, indels, etc.) associated with a particular disease (cancer, coagulopathies, etc.).
We use the NimbleGen SeqCap EZ protocol (Roche) for exome sequencing.
Personalized sequencing
After custom probe design, your regions of interest are enriched prior to sequencing which simplifies the subsequent data analysis. This protocol can be set up for any organism with an available reference genome.
GenoScreen's assets
- A dedicated team of scientists for each project.
- Comprehensive support with the elaboration of an experimental plan and project implementation.
- Assistance with data handling and interpretation of the results.
For a cost effective and responsive offer, GenoScreen proposes "Ready to Load" sequencing services for a wide range of libraries: a real research accelerator giving the opportunity to perform on last generation technical platform with advanced expertise.
Applications
"Ready-to-load" applications are available for mainly all of GenoScreen’s sequencing services:
- De novo sequencing or re-sequencing.
- RNA-Seq.
- Targeted sequencing.
- Metagenomics.
GenoScreen - Services provided
"Ready-to-load" sequencing is performed on latest-generation technical facilities: Illumina HiSeq®2500, MiSeq®, NextSeq®500 and HiSeq®4000 systems in paired-end or single-end runs of 50 to 250 bp.
GenoScreen performs quality control on the libraries with bioAnalyzer and fluorimetric assays. GenoScreen can also ensure the pooling of the sequencing libraries, if required.
We accept different types of librairies:
- TruSeq.
- Nextera(-XT).
- Amplicons.
- RADseq or ddRADseq with index and Illumina adaptors.
For the other types of librairies, please contact us.
The results
- Raw sequences (FastQ files) and quality control results after demultiplexing.
- Quality reports, including the number of reads obtained, the number of reads after application of quality filters, and the coverage obtained.
- The results are transferred to a secure FTP server or an external hard disk.
GenoScreen's assets
- Cutting-edge technical facilities.
- A top-of-the-range service at a keen price.
- Responsiveness.
High-throughput sequencing of the transcriptome (RNA-Seq) has revolutionized the quantitative and qualitative analysis of prokaryotic and eukaryotic organisms.
RNA-Seq significatively enables differential expression analyses:
- Measurement of the relative abundance of transcripts.
- Identification of the genes expressed differentially in various groups (tissues, treatments, etc.)
Applications
Transcriptome sequencing provides a complete, objective analysis of messenger RNAs:
- Differential expression: assessment of variations in the expression of several transcriptomes.
- Annotation of a transcriptome against a reference database.
- Identification of all specific sequences of the studied transcriptome.
- SNP analysis.
GenoScreen - Services provided
Two methods are used to enrich the RNA fraction of interest (mRNA) prior to cDNA synthesis and library preparation:
- Poly(A) capture (for eukaryotic total RNA).
- RNA depletion (via capture with complementary probes) and mRNA enrichment (for prokaryotic or eukaryotic total RNA).
Most of our RNA-Seq libraries are sequenced on Illumina® HiSeq®4000 systems.
GenoScreen's assets
- A dedicated team of scientists for each project.
- Assistance with elaborating the experimental plan and sequencing strategy.
- RNA extraction, quantification, purification, and quality control.
- Assistance with data management and interpretation of the results.
- Analysis of differential expression in the absence of a reference genome.
News
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Agenda
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April 2-3, 2024
Adebiotech - Innovations for plant health, Romainville-Paris - France