GenoScreen services
Services and Products
Expertise. Reliability. Precision.
Boost your research with genomics.
Predict tuberculosis resistance to anti-TB drugs
Deeplex® Myc-TB is an all-in-one test for species-level identification, genotyping and prediction of antibiotic resistance in Mycobacterium tuberculosis complex strains.
Exome sequencing
Exome sequencing allows targeting the genome coding regions. GenoScreen offers Whole Exome Sequencing (WES) services through the NimbleGen SeqCap EZ System (Roche).
Sanger Sequencing
GenoScreen's historical activity since 2001, Sanger sequencing is used in all fields and for a wide range of applications. It is the standard technique for sequencing DNA accurately and robustly. On our dedicated platform, we offer routine sequencing and, in addition, customised services with various levels of intervention and analysis.
Our Sanger sequencing platform
Equipped with ABI 3730XL capillary sequencers, our platform meets your one-off and regular sequencing needs from tubes, strips or 96-well plates containing your PCR products or vectors.
Molecular barcoding
Molecular barcoding is a technique for identifying and genetically characterising a sample by targeting a ubiquitous gene, often mitochondrial or chloroplast.
Applications:
- Identification of a species from an isolated sample (microorganism, animal or plant species)
- Comparison of several samples from the same species or closely related species
Services offered by GenoScreen
- DNA extraction from any type of matrix (optional)
- PCR amplification, sequencing and assignment of samples to databases (NCBI, BOLD, etc.)
- Construction of phylogenetic trees
- MLST typing and whole genome sequencing (WGS) to characterise the genome
- Solutions for complex matrices via metabarcoding
Discover our services in molecular barcoding
Additional Sanger services
Additional Sanger services
Primer walking
This sequencing method is ideal for:
Principle
The use of several primers makes it possible to obtain overlapping sequences leading to the reconstitution of the complete product. A universal primer is used to sequence an insert, obtaining a fragment of the vector sequence and the beginning of the insert.
A specific primer can then be designed on the sequence obtained and used to obtain the rest of the sequence. If the sequence of interest is known, all the sequencing reactions can be carried out simultaneously. This service can be performed either in single or double reading.
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Mutation detection
Sanger sequencing can be adopted to verify
Principle
Among other applications, the mutation detection is used to monitor TILLING (Targeting Induced Local Lesions in Genomes) in plants, or to verify mutations in genes of interest.
We can design specific primers to target a mutation and compare it to a reference sequence provided for verification.
If several mutations/positions are to be verified in the same genome region, we can also produce a detailed variance report compared with a reference gene.
Identification - Molecular barcoding
Molecular barcoding (DNA barcoding) is a molecular taxonomy technique used to identify and genetically characterise a sample from a ubiquitous gene, generally belonging to the ribosomal, mitochondrial or chloroplast genome (COI, 16S, rbcL, etc.).
DNA barcoding applications
DNA barcoding can be used to:
- Identify species from an isolated sample
- Compare several samples from the same species or closely related species (phylogeny)
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Identification by DNA barcoding
GenoScreen offers a complete molecular barcoding service and can handle your samples from DNA extraction onwards.
- Identification of isolated micro-organisms
- Identification of animal or plant species
For complex inital matrices (e.g. assemblies of bacterial populations), GenoScreen develops metabarcoding solutions using NGS amplicon sequencing to obtain screenings of samples: soil fauna, intestinal flora, coral reefs, etc.
GenoScreen offers you a straight-forward service for identifying your micro-organisms by DNA sequencing and comparison with international databases (NCBI or other).
For species identification, the ribosomal DNA regions are adapted to your micro-organism. In addition, other genomic targets (rpoB, gyrB, panC, etc.) can be used to refine this identification and adapt it to your requirements (for example, discrimination within a group of species sharing the same ribosomal DNA sequence).
Identification services
bacteria
fungi
yeast
microalgae
Workflow for isolated microorganisms
Extraction of your samples
from isolated colonies
PCR amplification on the appropriate genomic target
16S, 18S, 28S, ITS, ...
Forward/reverse
sequencing
Cleaning and assembly
in consensus, then comparison
Assignment
Our offer
Performed on :
- Agar (Petri dishes)
- Liquid culture plate
- FTA card or other matrix
From extracted DNA or thermolysate. We will compare your cleaned and assembled sequences with the international NCBI database.
Once the assignment has been completed, you will receive :
- Raw sequences and chromatograms (.seq/.ab1)
- Sequence consesus (.txt/.FA)
- Analysis report with homology percentages and assignment per sample
Additional solutions
In addition to identification, we are also able to produce phylogenetic trees according to your needs (comparison of samples with each other or within a panel of reference sequences).
Depending on your needs, we can also offer you other targets that are more specific to a particular genus, particularly for species that are too close genetically to the default target (for example, the panC target for the Bacillus genus).
GenoScreen also offers :
for strains whose species is known and for which a typing scheme is available
16S, 18S, 28S, ITS, ...
for more precise characterisation of your micro-organisms
GenoScreen offers you a straight-forward species identification service using DNA sequencing and comparison with international databases (NCBI and/or BOLD).
For species identification, we use target genes from the mitochondrial or chloroplast genome (COI, 16S, CYTB, rbcL, matK, etc.). We have universal primer pairs but we can also adapt to your project, using primers that are more specific to a genus or family depending on the literature.
Other target genes can be used to refine an identification or carry out a more complete phylogenetic study.
In addition to the identification, we are also able to produce phylogenetic trees according to your needs (comparison of samples with each other or with a panel of reference sequences).
Matrices for animal and plant studies
Whole insects or legs/heads
Extracted DNA
Hairs/feathers
Blood
Dried or fresh leaves
Tissues (muscle,organ, fin)
Swabs / FTA cards
Workflow applied to animals and plants
PCR amplification on the appropriate genomic target
COI, 16S, CYTB, rbcL, matK, ...
Forward/reverse
sequencing
Cleaning and assembly
in consensus, then comparison
Assignment
Our offer
PCR amplification on the various targets (6S, 18S, 28S, ITS, etc.) can be carried out using extracted DNA.
We will compare your cleaned and assembled sequences with the international NCBI (or Bold) database.
Once the assignment has been completed, you will receive :
- Raw sequences and chromatograms (.seq/.ab1)
- Consensus sequences (.txt/.FA)
- Analysis report with homology percentages and assignment per sample.
Complementary solutions
In addition to the identification, we can also generate phylogenetic trees according to your needs (comparison of samples with each other or with a panel of reference sequences).
GenoScreen also offers whole genome sequencing (WGS) for a more precise characterisation of your species.
Sequencing
GenoScreen is a major international player providing sequencing and genomic analysis services.
The company’s expertise is based on an unique experience in genomic analyses, the use of cutting-edge technologies and the development and supply of exclusive bioinformatics tools and R&D services. GenoScreen offers a very broad range of sequencing services, from the most basic to the most advanced.