Sequencing
Sanger Sequencing
GenoScreen's historical activity since 2001, Sanger sequencing is used in all fields and for a wide range of applications. It is the standard technique for sequencing DNA accurately and robustly. On our dedicated platform, we offer routine sequencing and, in addition, customised services with various levels of intervention and analysis.
Our Sanger sequencing platform
Equipped with ABI 3730XL capillary sequencers, our platform meets your one-off and regular sequencing needs from tubes, strips or 96-well plates containing your PCR products or vectors.
Molecular barcoding
Molecular barcoding is a technique for identifying and genetically characterising a sample by targeting a ubiquitous gene, often mitochondrial or chloroplast.
Applications:
- Identification of a species from an isolated sample (microorganism, animal or plant species)
- Comparison of several samples from the same species or closely related species
Services offered by GenoScreen
- DNA extraction from any type of matrix (optional)
- PCR amplification, sequencing and assignment of samples to databases (NCBI, BOLD, etc.)
- Construction of phylogenetic trees
- MLST typing and whole genome sequencing (WGS) to characterise the genome
- Solutions for complex matrices via metabarcoding
Discover our services in molecular barcoding
Additional Sanger services
Additional Sanger services
Primer walking
This sequencing method is ideal for:
Principle
The use of several primers makes it possible to obtain overlapping sequences leading to the reconstitution of the complete product. A universal primer is used to sequence an insert, obtaining a fragment of the vector sequence and the beginning of the insert.
A specific primer can then be designed on the sequence obtained and used to obtain the rest of the sequence. If the sequence of interest is known, all the sequencing reactions can be carried out simultaneously. This service can be performed either in single or double reading.
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Mutation detection
Sanger sequencing can be adopted to verify
Principle
Among other applications, the mutation detection is used to monitor TILLING (Targeting Induced Local Lesions in Genomes) in plants, or to verify mutations in genes of interest.
We can design specific primers to target a mutation and compare it to a reference sequence provided for verification.
If several mutations/positions are to be verified in the same genome region, we can also produce a detailed variance report compared with a reference gene.
Identification - Molecular barcoding
Molecular barcoding (DNA barcoding) is a molecular taxonomy technique used to identify and genetically characterise a sample from a ubiquitous gene, generally belonging to the ribosomal, mitochondrial or chloroplast genome (COI, 16S, rbcL, etc.).
DNA barcoding applications
DNA barcoding can be used to:
- Identify species from an isolated sample
- Compare several samples from the same species or closely related species (phylogeny)
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Identification by DNA barcoding
GenoScreen offers a complete molecular barcoding service and can handle your samples from DNA extraction onwards.
- Identification of isolated micro-organisms
- Identification of animal or plant species
For complex inital matrices (e.g. assemblies of bacterial populations), GenoScreen develops metabarcoding solutions using NGS amplicon sequencing to obtain screenings of samples: soil fauna, intestinal flora, coral reefs, etc.
GenoScreen offers you a straight-forward service for identifying your micro-organisms by DNA sequencing and comparison with international databases (NCBI or other).
For species identification, the ribosomal DNA regions are adapted to your micro-organism. In addition, other genomic targets (rpoB, gyrB, panC, etc.) can be used to refine this identification and adapt it to your requirements (for example, discrimination within a group of species sharing the same ribosomal DNA sequence).
Identification services
bacteria
fungi
yeast
microalgae
Workflow for isolated microorganisms
Extraction of your samples
from isolated colonies
PCR amplification on the appropriate genomic target
16S, 18S, 28S, ITS, ...
Forward/reverse
sequencing
Cleaning and assembly
in consensus, then comparison
Assignment
Our offer
Performed on :
- Agar (Petri dishes)
- Liquid culture plate
- FTA card or other matrix
From extracted DNA or thermolysate. We will compare your cleaned and assembled sequences with the international NCBI database.
Once the assignment has been completed, you will receive :
- Raw sequences and chromatograms (.seq/.ab1)
- Sequence consesus (.txt/.FA)
- Analysis report with homology percentages and assignment per sample
Additional solutions
In addition to identification, we are also able to produce phylogenetic trees according to your needs (comparison of samples with each other or within a panel of reference sequences).
Depending on your needs, we can also offer you other targets that are more specific to a particular genus, particularly for species that are too close genetically to the default target (for example, the panC target for the Bacillus genus).
GenoScreen also offers :
for strains whose species is known and for which a typing scheme is available
16S, 18S, 28S, ITS, ...
for more precise characterisation of your micro-organisms
GenoScreen offers you a straight-forward species identification service using DNA sequencing and comparison with international databases (NCBI and/or BOLD).
For species identification, we use target genes from the mitochondrial or chloroplast genome (COI, 16S, CYTB, rbcL, matK, etc.). We have universal primer pairs but we can also adapt to your project, using primers that are more specific to a genus or family depending on the literature.
Other target genes can be used to refine an identification or carry out a more complete phylogenetic study.
In addition to the identification, we are also able to produce phylogenetic trees according to your needs (comparison of samples with each other or with a panel of reference sequences).
Matrices for animal and plant studies
Whole insects or legs/heads
Extracted DNA
Hairs/feathers
Blood
Dried or fresh leaves
Tissues (muscle,organ, fin)
Swabs / FTA cards
Workflow applied to animals and plants
PCR amplification on the appropriate genomic target
COI, 16S, CYTB, rbcL, matK, ...
Forward/reverse
sequencing
Cleaning and assembly
in consensus, then comparison
Assignment
Our offer
PCR amplification on the various targets (6S, 18S, 28S, ITS, etc.) can be carried out using extracted DNA.
We will compare your cleaned and assembled sequences with the international NCBI (or Bold) database.
Once the assignment has been completed, you will receive :
- Raw sequences and chromatograms (.seq/.ab1)
- Consensus sequences (.txt/.FA)
- Analysis report with homology percentages and assignment per sample.
Complementary solutions
In addition to the identification, we can also generate phylogenetic trees according to your needs (comparison of samples with each other or with a panel of reference sequences).
GenoScreen also offers whole genome sequencing (WGS) for a more precise characterisation of your species.
Sequencing
GenoScreen is a major international player providing sequencing and genomic analysis services.
The company’s expertise is based on an unique experience in genomic analyses, the use of cutting-edge technologies and the development and supply of exclusive bioinformatics tools and R&D services. GenoScreen offers a very broad range of sequencing services, from the most basic to the most advanced.
Sanger sequencing
GenoScreen Sanger sequencing services can be adapted to any type of project: magnitudes, delays and production frequencies, for a reading between 100 and 1200 bp.
Next-generation sequencing
GenoScreen offers a complete range of Next Generation Sequencing (NGS) services updated with the latest technologies (Illumina, PacBio ...).
NGS - High-power genomics for the life sciences
Since 2008, GenoScreen offers a full range of next-generation sequencing (NGS) services in application to genomes, metagenomes and transcriptomes.
GenoScreen has cutting-edge technical facilities, giving you access to the latest technologies (Illumina, PacBio, etc.).
GenoScreen - Services provided
- Whole-genome sequencing (WGS)
New sequencing technologies available at GenoScreen offer the opportunity to analyse the entire primary structure of an isolated organism genome, whether eukaryotic, prokaryotic or viral. The analysis of data obtained allows to precisely characterize and describe any type of organisation and thus responds to a large panel of research problems. - Metagenomics
Metagenomics molecular approaches allows to analyse a community of organisms as a whole and to study a complex ecological niche exhaustively: a powerful and innovative approach to characterize the genes and, therefore, the biological functions carried by these microbiomes. - Targeted sequencing
Targeted sequencing allows specific analysis of targeted genes (or gene regions) on many samples in parallel. With NGS sequencing on MiSeq®, NextSeq500® or Hiseq® platforms, multiple genes can be evaluated with a smaller data volume, which is easier to manage and analyse than WGS datas. - Transcriptomics
High-throughput transcriptome sequencing (RNA-seq) has revolutionised prokaryotic and eukaryotic organisms quantitative and qualitative analyses. It allows, in particular, to perform differential expression analyses. - Ready to load
For a cost effective and responsive offer, GenoScreen proposes "Ready to Load" sequencing services for a wide range of libraries: a real research accelerator giving the opportunity to perform on last generation technical platform with advanced expertise.
The GenoScreen's assets
- A full range of NGS services
- Expert analysis and advice on the design of each analysis
- Unique expertise in microbiology
- High-quality data
- Exclusive bioinformatics tools: assembly, annotation, SNP detection, etc.
- Guaranteed short delay time
Sanger sequencing - The low-throughput genomics solution
Sanger sequencing is a well-proven, low-throughput analytical solution that is well suited to fragments of all sizes (100-1200 bp read length). GenoScreen’s Sanger sequencing services can be adapted to suit all types of project, regardless the size, delay time or frequency.
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Sanger sequencing applications
Despite the emergence of new generation technologies (NGS), Sanger sequencing remains a reference method in many fields thanks to its reliability and accuracy.
GenoScreen offer
Flexible and available, our teams carry out sequencing from sample preparation to data analysis. See our documentation below to find out more about our services and to prepare your sequencing request form.
User manual
Form (with purification)
Form (without purification)
Premium range
The Premium offers correspond to a complete and personalised analysis of samples:
- Adaptation of the protocol to GC-rich sequences
- Supply of universal primers
- Sequence reading up to 1200 bp
- Results sent within 24 to 48 hours
The Premium range gives you access to our hotline, by phone and email: expert scientific support for sequence profile analysis.
Workflow tailored to your needs
Purification and dosing
Sequencing reactions
Purification and migration on capillary sequencer
Deliverables with different levels of analysis
After-sales service, eventual reprocessing with optimised protocol
One-Shot
Free purification
Deliverables
.seq / .ab1
Monthly activity report (including remaining credits)
One-Shot Plus
Deliverables
+
Semi-automated review of sequences and corrections with IUPAC annotations
Optimized
Deliverables
+
Manual IUPAC annotations
Reprocessing with optimised protocol (on request)
One-Shot
Purification gratuite
Livrables
.seq / .ab1
Rapport mensuel d'activité (incluant les crédits restants)
One-Shot Plus
Livrables
+
Relecture des séquences et corrections avec annotations IUPAC semi-automatisée
Optimized
Livrables
+
Annotations IUPAC manuelle
Rapport QC (Quality control) relecture manuelle et commentée de chaque séquence avec score qualité
Retraitement avec protocole optimisé (sur demande)
All our offers are open to different sample formats:
- tubes
- strips
- plates, complete or not
Our One-Shot Plus and Optimized offers include additional information:
- Signal optimisation
- Reduction of any backgroun noise
- Assignment of indeterminate bases
- A specific protocol for mutation detection
Additional analyses
GenoScreen offers a range of services to complement sequencing:
Economic range
All the quality and responsiveness of GenoScreen at a low price!
Using your own PCR product + primer mix or even the sequencing reaction you have produced, we can take your samples to a more advanced stage for an optimised price.
GenoScreen advantages
- Flexible sequencing offer that adapts to your needs
-
Personalised monitoring of your projects
-
Simple ordering system
-
Monthly invoicing or prepaid account
-
Free transport of your samples by a GenoScreen-approved operator
-
Optimised protection of samples in Genobox
Metagenomics - Powerful analyses of microbial communities
At GenoScreen, we turn metagenomic data into actionable insights through tailored analyses and cutting-edge scientific support.
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Why choose metagenomics?
Metagenomics allows the analysis of the diversity and functioning of microbial communities directly in their environment, without prior culturing. It offers a deep understanding of the composition, interactions, and functions of the microbiome, paving the way for major applications in human and animal health, dermocosmetics, environmental studies, and industrial biotechnologies.
in genomics &
metagenomics
Metagenomics: Our Expertise
Our experience has allowed us to develop and refine robust methodologies tailored to the uniqueness of each sample type, ensuring the relevance and reliability of your results
You will be supported by a multidisciplinary team of experts in bioinformatics and microbiology, ensuring comprehensive and personalized project support.
Quality assurance, the core of our approach
We have implemented a systematic and thorough internal quality control system to validate the performance of our protocols and ensure the integrity of your results.
You will benefit from clear and interactive results, designed for in-depth understanding and optimal use of your data.
rigorous &
traceable analyses
Methodologies adapted to every sample type
Each biological or environmental matrix is unique and requires a specific approach. Through our expertise, we have developed optimized protocols for various matrices:
Other sample types? Our experience allows us to adapt our methods to a wide variety of samples.
Let’s talk about it!
Our workflow applied to metagenomics
Optimized DNA extraction for each matrix
stool, soil, skin, fermented samples, etc.
Library preparation
with integrated quality controls
High-throughput sequencing adapted to your needs
Bioinformatics analysis
Interpretation & reporting
More technologies, more personalisation
We offer DNA sequencing technologies fully tailored to your analytical goals : Illumina (short-read) for high-throughput, accurate sequencing, and PacBio (long-read) for high-resolution insights, ideal for detailed microbial genome characterization.
Depending on the level of analysis required, our protocols are available in metabarcoding (16S, 18S, ITS) for a targeted approach, or shotgun metagenomics for a comprehensive exploration of microbial diversity, both taxonomic and functional.
Additionally, sequencing depth is adjusted to your specific needs, taking into account the richness and complexity of the microbiota under study(whether human, animal, plant, or environmental in origin).
The GenoScreen experience in a nutshell...
From setting up your project to delivering the results
Methods and solutions tailored to your projects
Micro-organism studies for over 20 years
Support beyond results delivery
‘materials and methods’ explained