GenoScreen is now a certified service provider of Oxford Nanopore Technologies.
GenoScreen is proud to be the third French service provider certified by Oxford Nanopore Technologies. This certification ensures the highest quality standards for long-read sequencing projects on the GridION sequencing platform.
Find out how GenoScreen's long-read sequencing solutions can assist you in your genomics projects:
- Characterization of modified bacterial strains
Get a precise analysis of the content of your strains of industrial, medical or agri-food interest. Our analyses have been specifically designed for the completion of regulatory files such as ANSES/EFSA for genetically modified microorganisms but can be adapted to all your regulatory needs. (https://efsa.onlinelibrary.wiley.com/doi/full/10.2903/j.efsa.2021.6506)
- Complete de novo assembly of parental and modified strains.
- Genetic comparison (SNP/Indel/structural variants) between parental and engineered strains with a complete catalog of the modifications including:
- Insertion site and copy number of inserted sequences.
- Confirmation of sequence/gene deletions.
- Search for antibiotic resistance genes.
- Search for virulence factors.
- Transparency and methodological details from DNA extraction to data analysis.
- Generation of reports following EFSA recommendations for the characterization of genetically modified microorganisms.
- Generation of complete genomes
- The GenoRef solution allows, via a manually assisted assembly by our bioinformaticians, to obtain a high-quality reference genome for:
- A micro-organism with no reference genome.
- Enabling high quality comparative analysis (e.g. search for variants).
- Obtain a clear and complete view of your genomes of interest.
- Characterize genomic differences between related strains.
- An interactive report to get a complete view of the quality of your assemblies at a glance.
- Detection of structural variants
Structural variants (variants of more than 50 bp) are often overlooked in comparative genomics studies, which often focus on point mutations such as SNVs and Indels. These variants include insertions, deletions, duplications, inversions and translocations. Their size can be extremely variable size and affect from a few tens of base pairs up to entire chromosomes.
- Gain confidence in the detection of such events (compared to short-read sequencing-based approach).
- In one round of sequencing, get a reference grade level assembly, characterize all variants in your sample (SNVs, Indels, Structural variants).
- And many other applications...
Direct RNA sequencing (without cDNA intermediary), Epigenetics, Adaptive sampling, Large amplicons, etc.