High-throughput sequencing of the transcriptome (RNA-Seq) has revolutionized the quantitative and qualitative analysis of prokaryotic and eukaryotic organisms.
RNA-Seq significatively enables differential expression analyses:
- Measurement of the relative abundance of transcripts.
- Identification of the genes expressed differentially in various groups (tissues, treatments, etc.)
Transcriptome sequencing provides a complete, objective analysis of messenger RNAs:
- Differential expression: assessment of variations in the expression of several transcriptomes.
- Annotation of a transcriptome against a reference database.
- Identification of all specific sequences of the studied transcriptome.
- SNP analysis.
GenoScreen - Services provided
Two methods are used to enrich the RNA fraction of interest (mRNA) prior to cDNA synthesis and library preparation:
- Poly(A) capture (for eukaryotic total RNA).
- RNA depletion (via capture with complementary probes) and mRNA enrichment (for prokaryotic or eukaryotic total RNA).
Most of our RNA-Seq libraries are sequenced on Illumina® HiSeq®4000 systems.
- A dedicated team of scientists for each project.
- Assistance with elaborating the experimental plan and sequencing strategy.
- RNA extraction, quantification, purification, and quality control.
- Assistance with data management and interpretation of the results.
- Analysis of differential expression in the absence of a reference genome.