Targeted sequencing allows to analyze specific genome regions regardless size, continuity or discontinuity. By reducing sequenced area thanks to targeted enrichment or amplification, this approach allows you to perform braod studies. These studies can be led on individuals populations, as for example in the framework of association studies, for genetic markers identification linked to pathologies.
Thanks to its experience, our team will advise you on the best combination of technologies for custom enrichment steps (Nimblegen, Agilent), or targeted amplification (Haloplex, Raindance, Multiplicon) with GsFLX / or GsJunior HiSeq2500/MiSeq sequencers. This combination will be developped according to the quantity of samples to analyze and the size of your area of interest.
Exome sequencing allows you to analyze the coding regions and UTR sequences of samples of your cohorts to identify common or rare changes : SNPs, mutations and indels within your association studies, epidemiological studies , clinical trials, pharmacogenetics and pharmacogenomics.
With it expertise in the field of human genetics, our team will provide you with a quality service by combining TruSeq capture technologies (Illumina) or V5/V5 + UTR SureSelect (Agilent) with HiSeq2500 technology (Illumina). Our offer includes the analysis of your data using a standardized bioinformatic pipeline developed in-house. This pipeline allows you to receive a list of common or rare events identified in the samples.
ChipSeq sequencing aims to isolate regions of a genome that are involved in gene regulation. They contain consensus sequences on which transcription factor acts to favor or inhibit the expression of a gene.
Knowing where these consensus sites are located, and which genes they are associated with gives information regarding the site of action of the transcription factor in the genome.