Microsatellites libraries and Genotyping
Production of microsatellites libraries
by Next Generation Sequencing
- A standardized method
Based on high throughput sequencing, this technique* has been validated on more than 400 different species sequenced by Genoscreen.
- An unequalled efficiency
From 1 µg of genomic DNA, Geno Sat® allows to obtain at least 8 000 raw sequences, 2000 sequences containing microsatellites motifs and 100 bioinformatically validated primers pairs, all in a record time of 2 months.
- Ready-to-publish data
According to your needs, Genoscreen proposes a simple, attractive and flexible solution declined in 3 options:
> GENO SAT®
Production of microsatellites markers' library by high throughput pyrosequencing (GS FLX®, Roche Diagnostics®). Obtention of a minimum of 100 bioinformatically validated primers pairs, framing microsatellites motives.
> GENO SAT® PACK
Biological validation of primers pairs generated thanks to Geno Sat® library by PCR
and loading on agarose gel
Polymorphism study of primers pairs biologically validated with labelled markers (6FAM/VIC/NED/PET) and fragments analysis on capillary sequencer (3730XL®, Applied Biosystems®)
> GENO SAT® PACK PLUS: Microsatellites Genotyping
GENO SAT® PACK
Genotyping on a large population of samples representing the intra-specific genetic variability
of your species using polymorphic microsatellites markers
We propose the definition of the optimal conditions for microsatellites analysis:
- Multiplexed PCR optimization and pool of PCR products before migration on capillary sequencer
- Genotyping of samples on capillary sequencer and analysis of associated genotypes - ladder included (GeneScan LIZ 500)
- Available combinations of fluorophores: 6FAM-VIC-NED-PET
- Storage of DNA samples during 4 weeks after the results report
- Confidentiality of information provided and obtained results
We can also take over every single step of our offer individually (Biological validation / Polymorphism study / Development of multiplex / Genotyping of populations with or without analysis)
* Process GENOSCREEN – INRA – Montpellier SUPAGRO– Provence university.
>> Customer publications
V. Lesieur, M. Jeanneau, J. F. Martin, M. C. Bon
Development and characterization of 11 microsatellite markers in the root-gall-forming weevil, Ceutorhynchus assimilis (Coleoptera: Curculionidae)
Dima Souleman, Fabien Grumiaux, Helene Frerot, Franck Vandenbulcke, Maxime Pauwels
Isolation and characterization of eight polymorphic microsatellites markers for the earthworm Lumbricus terrestris
Mieuzet Lucie, A. Quillévéré, M.L. Pilet, C. Le May
Development and characterization of microsatellite markers for the oomyceta Aphanomyces euteiches
>> Genoscreen publications
Martin JF, Pech N, Meglécz E, Ferreira S, Costedoat C, Dubut V, Malausa T, Gilles A
Representativeness of microsatellite distributions in genomes, as revealed by 454 GS-FLX Titanium pyrosequencing.
BMC Genomics 2010.
André Gilles, Emese Meglécz, Nicolas Pech, Stéphanie Ferreira, Thibaut Malausa and Jean-François Martin
Accuracy and quality assessment of 454 GS-FLX Titanium pyrosequencing
BMC Genomics 2011