{"id":111,"date":"2017-12-14T18:29:28","date_gmt":"2017-12-14T17:29:28","guid":{"rendered":"https:\/\/www.genoscreen.fr\/genotypage-de-snp\/"},"modified":"2026-06-05T20:51:22","modified_gmt":"2026-06-05T18:51:22","slug":"genotypage-des-snp","status":"publish","type":"page","link":"https:\/\/www.genoscreen.fr\/fr\/services-genomiques\/genotypage-des-snp\/","title":{"rendered":"G\u00e9notypage de SNP"},"content":{"rendered":"\n

Cette technique repose sur la variation d\u2019un seul nucl\u00e9otide<\/strong> \u00e0 une position donn\u00e9e (Single Nucleotide Polymorphism) pouvant notamment entra\u00eener des modifications d’expression de g\u00e8nes<\/strong>. En proposant des analyses pouvant porter sur le g\u00e9nome entier, GenoScreen vous accompagne afin de rep\u00e9rer des associations inconnues g\u00e9notype-ph\u00e9notype<\/strong> ou pour cibler des variations g\u00e9n\u00e9tiques<\/strong> d\u00e9j\u00e0 connues.<\/p>\n\n\n\n

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Discutons de votre projet<\/a><\/div>\n<\/div>\n\n\n\n

Dans quels contextes pratiquer un g\u00e9notypage de SNPs ?<\/h2>\n\n\n\n

En raison de leur pr\u00e9sence sur tout type de g\u00e9nome, le g\u00e9notypage de SNPs<\/strong> peut avoir un int\u00e9r\u00eat dans de nombreux domaines d’application. Habitu\u00e9e \u00e0 intervenir sur des probl\u00e9matiques tr\u00e8s diversifi\u00e9es, notre \u00e9quipe pourra vous assister dans le choix de la meilleure technique pour r\u00e9pondre \u00e0 vos besoins<\/strong>.<\/p>\n\n\n\n\n\n\n

Les offres GenoScreen<\/h2>\n\n\n\n

Nos prestations s’adaptent \u00e0 vos besoins. Nous pouvons utiliser les essais d\u00e9j\u00e0 disponibles<\/strong> dans les bases de donn\u00e9es, ou identifier et cibler des SNPs d’int\u00e9r\u00eat<\/strong> \u00e0 partir de donn\u00e9es de s\u00e9quen\u00e7age (avec ou sans g\u00e9nome de r\u00e9f\u00e9rence).<\/p>\n\n\n\n\n\n\n

G\u00e9notypage par qPCR<\/h3>\n\n\n\n

La qPCR permet d\u2019identifier des variations g\u00e9n\u00e9tiques comme les SNPs<\/strong> en amplifiant sp\u00e9cifiquement des s\u00e9quences d\u2019ADN. Ce g\u00e9notypage repose sur des m\u00e9thodes comme les sondes TaqMan<\/strong>, qui offrent une discrimination pr\u00e9cise des all\u00e8les, ou la PCR comp\u00e9titive KASPar<\/strong>, plus \u00e9conomique pour les analyses \u00e0 grande \u00e9chelle ou r\u00e9currentes.<\/p>\n\n\n\n

Ces deux m\u00e9thodes d’identification des variantions g\u00e9n\u00e9tiques op\u00e9r\u00e9es par GenoScreen vous seront propos\u00e9s selon la finalit\u00e9 et la r\u00e9currence de votre projet. Notre \u00e9quipe peut \u00e9galement vous accompagner pour l’\u00e9laboration d’un kit de g\u00e9notypage.<\/p>\n\n\n\n\n\n\n

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Une approche flexible \u00e0 vos besoins<\/h4>\n\n\n\n

Nous pr\u00e9voyons avec vous l’ensemble des \u00e9tapes n\u00e9cessaires pour mener \u00e0 bien votre projet, quelles que soient vos contraintes de nombre d’\u00e9chantillons ou de cibles, de d\u00e9lai et de co\u00fbt.<\/p>\n<\/div>\n\n\n\n

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\"Recherche,<\/figure>\n<\/div>\n<\/div>\n\n\n\n

G\u00e9notypage par s\u00e9quen\u00e7age<\/h3>\n\n\n\n
\n
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\"illustration<\/figure>\n<\/div>\n\n\n\n
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 <\/p>\n\n\n\n

Ces technologies sont utilis\u00e9es couramment dans des projets de g\u00e9n\u00e9tique de populations, biologie de la conservation ou d\u2019\u00e9cologie<\/strong>. Il n\u2019est pas n\u00e9cessaire d\u2019avoir acc\u00e8s \u00e0 un g\u00e9nome de r\u00e9f\u00e9rence pour effectuer l\u2019analyse.<\/p>\n\n\n\n

Les donn\u00e9es obtenues peuvent \u00eatre utilis\u00e9es pour identifier des SNPs en tant que marqueurs g\u00e9n\u00e9tiques<\/strong>, faire des analyses de structure des populations<\/strong> et\/ou phylog\u00e9n\u00e9tiques<\/strong>.<\/p>\n<\/div>\n<\/div>\n\n\n\n\n\n\n

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Contactez-nous pour en savoir plus<\/a><\/div>\n<\/div>\n\n\n\n

Sanger<\/h3>\n\n\n\n

En raison de sa fiabilit\u00e9 et de sa pr\u00e9cision<\/strong>, le s\u00e9quen\u00e7age Sanger reste la m\u00e9thode de r\u00e9f\u00e9rence lorsqu’il s’agit de v\u00e9rifier des r\u00e9sultats incertains de qPCR ou confirmer des mutations observ\u00e9es par s\u00e9quen\u00e7age haut d\u00e9bit.<\/p>\n","protected":false},"excerpt":{"rendered":"

Cette technique repose sur la variation d\u2019un seul nucl\u00e9otide \u00e0 une position donn\u00e9e (Single Nucleotide Polymorphism) pouvant notamment entra\u00eener des modifications d’expression de g\u00e8nes. En proposant des analyses pouvant porter sur le g\u00e9nome entier, GenoScreen vous accompagne afin de rep\u00e9rer des associations inconnues g\u00e9notype-ph\u00e9notype ou pour cibler des variations g\u00e9n\u00e9tiques d\u00e9j\u00e0 connues. Dans quels contextes pratiquer […]<\/p>\n","protected":false},"author":1,"featured_media":1043,"parent":2200,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"page-categories":[155],"class_list":["post-111","page","type-page","status-publish","has-post-thumbnail","hentry","page-categories-genoscreen-services"],"yoast_head":"\nG\u00e9notypage de SNP - GenoScreen<\/title>\n<meta name=\"description\" content=\"GenoScreen propose des services de g\u00e9notypage performants, bas\u00e9s sur l'analyse ou la d\u00e9couverte de SNP, pouvant porter sur le g\u00e9nome entier.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.genoscreen.fr\/fr\/services-genomiques\/genotypage-des-snp\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"G\u00e9notypage de SNP - 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