{"id":699,"date":"2023-09-15T10:07:55","date_gmt":"2023-09-15T08:07:55","guid":{"rendered":"https:\/\/www.genoscreen.fr\/brucella-mlva-16-typing-solution\/"},"modified":"2026-07-13T19:28:54","modified_gmt":"2026-07-13T17:28:54","slug":"brucella","status":"publish","type":"page","link":"https:\/\/www.genoscreen.fr\/en\/genomic-services\/brucella\/","title":{"rendered":"Brucella MLVA-16 typing solution"},"content":{"rendered":"\n<p class=\"intro wp-block-paragraph\">The <strong>Brucella MLVA-16<\/strong> typing solution, developed by <a href=\"https:\/\/www.genoscreen.fr\/en\/\">GenoScreen<\/a>, enables health authorities to effectively monitor and control cases of brucellosis in both animals and humans as well as <strong><em>Ochrobactrum<\/em> <\/strong>infections.<\/p>\n\n<p class=\"wp-block-paragraph\">Conventional diagnostic techniques struggle to distinguish between <em>Brucella <\/em>or <em>Ochrobactrum <\/em>strains due to their close relatedness, leading to a suboptimal disease management<sup>1<\/sup>.<\/p>\n\n<p class=\"wp-block-paragraph\"><em>Brucella <\/em>species are not known to survive long in open environments, their exhibit various host tropisms and varying levels of pathogenicity, which can result in <strong>brucellosis<\/strong>, a potentially fatal disease in both humans and animals. In contrast, <em>Ochrobactrum <\/em>species are commonly found in the environment, are less pathogenic and require a different treatment approach for successful eradication<sup>1<\/sup>.  <\/p>\n\n<p class=\"wp-block-paragraph\">The <strong>Brucella MLVA-16<\/strong> typing solution offers a valuable tool for accurately identifying and distinguishing species within both the <em>Brucella <\/em>and <em>Ochrobactrum <\/em>genera. This tool enables the effective tracking and management of infections caused by these organisms. <\/p>\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-04478499 wp-block-buttons-is-layout-flex\" style=\"margin-top:20px;margin-bottom:20px\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link has-elementor-28-fc-130-color has-elementor-primary-background-color has-text-color has-background has-link-color wp-element-button\" href=\"https:\/\/www.genoscreen.fr\/en\/genomic-services\/request-a-quote\/\" style=\"border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-left-radius:0px;border-bottom-right-radius:0px\">Request a quote<\/a><\/div>\n<\/div>\n\n<h2 class=\"wp-block-heading\">The Brucella MLVA-16 Workflow<\/h2>\n\n<p class=\"wp-block-paragraph\">The <strong>Brucella MLVA-16<\/strong> typing solution measures the length of 16 genetic loci with Variable Number of Tandem Repeats (VNTR) to perform a <strong>Multiple Loci VNTR Analysis (MLVA)<\/strong>, and to assign to each result a MLVA numerical code specific to <em>Brucella <\/em>or <em>Ochrobactrum <\/em>species\/strains<sup>2<\/sup>.<\/p>\n\n<ul class=\"wp-block-list\">\n<li>The <strong>Brucella MLVA-16<\/strong> typing solution is available as a service and as a kit.<\/li>\n\n\n\n<li>The kit contains 4 <strong>PCR <\/strong>mixes with fluorochrome-labeled primers, each amplifying 4 VNTR (16 VNTR in total) from purified <strong>DNA <\/strong>extracted. <\/li>\n\n\n\n<li>The <strong>MLVA <\/strong>profile can be compared with a database that enables strain identification and tracking.<\/li>\n\n\n\n<li>Fragment sizes are converted bioinformatically into a succession of numbers of repeats for each VNTR, defining a MLVA numerical code specific to <em>Brucella <\/em>or <em>Ochrobactrum <\/em>species\/strains.<\/li>\n\n\n\n<li>The PCR products are migrated on a capillary sequencer for fragment size determination.<\/li>\n<\/ul>\n\n<h2 class=\"wp-block-heading\">Brucellosis, a \u201cOne Health\u201d challenge<\/h2>\n\n<p class=\"wp-block-paragraph\"><strong>Brucellosis <\/strong>(or <strong>Malta fever<\/strong>) is one of the most widespread animal disease transmissible to humans (zoonose) and the first cause of contamination in laboratories. The <strong>bacteria <\/strong>are transmitted to humans through direct contact with infected animals, ingestion of contaminated animal products or inhalation of aerosols3. <\/p>\n\n<p class=\"wp-block-paragraph\">Animal brucellosis has a broad impact, affecting mammals across the board, with notable consequences in cattle, sheep, and goats. In the agricultural sector, the disease often leads to issues such as abortions, infertility, reduced milk production, resulting in diminished productivity and substantial economic losses for livestock farmers<sup>4<\/sup>. <\/p>\n\n<p class=\"wp-block-paragraph\">Human brucellosis, on the other hand, presents a pressing public health issue. It manifests with severe and enduring flu-like symptoms that can, in some cases, progress into chronic forms. These chronic forms may entail various complications, including joint problems, cardiac ailments, neurological symptoms, and more<sup>3<\/sup>. In 2023, the incidence of human brucellosis is estimated at over 1.6 &#8211; 2.1 million cases per year<sup>5<\/sup>.  <\/p>\n\n<p class=\"wp-block-paragraph\">In contrast to high-income countries where effective &#8220;test-and-slaughter&#8221; policies have nearly eliminated brucellosis in infected herds, low- and middle-income countries continue to grapple with the burden of this disease. Moreover, the increasing global migrations of both humans and animals, along with more frequent interactions between them, has heightened the risk of <em>Brucella <\/em>infections. This risk includes now the emergence of newly discovered pathogenic strains that may elude existing surveillance programs, posing a worldwide threat to disease containment efforts<sup>3<\/sup>.  <\/p>\n\n<h2 class=\"wp-block-heading\">References:<\/h2>\n\n<ul class=\"wp-block-list\">\n<li>Moreno, Edgardo et al. (2023). \u201cIf You&#8217;re Not Confused, You&#8217;re Not Paying Attention: <em>Ochrobactrum<\/em> Is Not Brucella.\u201d <em>Journal of clinical microbiology<\/em> 61(8). <a href=\"https:\/\/doi.org\/10.1128\/jcm.00438-23\">https:\/\/doi.org\/10.1128\/jcm.00438-23<\/a> <\/li>\n\n\n\n<li>Al Dahouk, Sascha et al. (2007). \u201cEvaluation of Brucella MLVA typing for human brucellosis.\u201d <em>Journal of microbiological methods<\/em> 69(1) 137-45. <a href=\"https:\/\/doi.org\/10.1016\/j.mimet.2006.12.015\">https:\/\/doi.org\/10.1016\/j.mimet.2006.12.015<\/a> <\/li>\n\n\n\n<li><a href=\"https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/brucellosis\">https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/brucellosis<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.woah.org\/en\/disease\/brucellosis\/\">https:\/\/www.woah.org\/en\/disease\/brucellosis\/<\/a><\/li>\n\n\n\n<li>Laine, C. G., Johnson, V. E., Scott, H. M., &amp; Arenas-Gamboa, A. M. (2023). Global Estimate of Human Brucellosis Incidence. <em>Emerging infectious diseases<\/em>, <em>29<\/em>(9), 1789\u20131797. <a href=\"https:\/\/doi.org\/10.3201\/eid2909.230052\">https:\/\/doi.org\/10.3201\/eid2909.230052<\/a> <\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>The Brucella MLVA-16 typing solution, developed by GenoScreen, enables health authorities to effectively monitor and control cases of brucellosis in both animals and humans as well as Ochrobactrum infections. Conventional diagnostic techniques struggle to distinguish between Brucella or Ochrobactrum strains due to their close relatedness, leading to a suboptimal disease management1. Brucella species are not [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":2351,"parent":2225,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"page-categories":[158],"class_list":["post-699","page","type-page","status-publish","has-post-thumbnail","hentry","page-categories-genoscreen-services"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Brucella MLVA-16 typing solution - GenoScreen<\/title>\n<meta name=\"description\" content=\"The Brucella MLVA-16 typing solution, developed by GenoScreen, enables public health authorities to effectively monitor and control cases of brucellosis in animals and humans, as well as Ochrobactrum infections.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.genoscreen.fr\/en\/genomic-services\/brucella\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Brucella MLVA-16 typing solution - 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