{"id":182,"date":"2016-02-15T10:33:01","date_gmt":"2016-02-15T09:33:01","guid":{"rendered":"https:\/\/www.genoscreen.fr\/targeted-sequencing-an-in-depth-analysis-of-regions-of-interest\/"},"modified":"2026-06-07T16:57:31","modified_gmt":"2026-06-07T14:57:31","slug":"targeted-sequencing","status":"publish","type":"page","link":"https:\/\/www.genoscreen.fr\/en\/genomic-services\/sequencing\/next-generation-sequencing-ngs\/targeted-sequencing\/","title":{"rendered":"Targeted sequencing &#8211; An in-depth analysis of regions of interest"},"content":{"rendered":"\n<p class=\"intro wp-block-paragraph\">Targeted sequencing allows specific analysis of targeted genes (or gene regions) on many samples in parallel. With <a href=\"https:\/\/www.genoscreen.fr\/en\/genomic-services\/sequencing\/next-generation-sequencing-ngs\/\" type=\"page\" id=\"106\">NGS sequencing<\/a> on <a title=\"Genoscreen, partenaire d'Illumina\" href=\"https:\/\/emea.illumina.com\/systems\/sequencing-platforms\/miseq.html\">MiSeq\u00ae<\/a>, NextSeq500\u00ae or Hiseq\u00ae platforms, multiple genes can be evaluated with a smaller data volume, which is easier to manage and analyse than WGS datas.<\/p>\n\n<h2 class=\"wp-block-heading\">Applications<\/h2>\n\n<p class=\"wp-block-paragraph\">Sequencing or targeted resequencing can achieve very specific objectives:<\/p>\n\n<ul class=\"wp-block-list\">\n<li>Screening of genomic regions of interest.<\/li>\n\n\n\n<li>Complete coverage of <strong>coding regions<\/strong>, with a limited volume of <strong>genomic data<\/strong>.<\/li>\n\n\n\n<li>Analysis of microbial genes.<\/li>\n<\/ul>\n\n<h2 class=\"wp-block-heading\">GenoScreen &#8211; Services provided<\/h2>\n\n<h3 class=\"wp-block-heading\">Amplicon sequencing<\/h3>\n\n<p class=\"wp-block-paragraph\">A number of the target regions are amplified by PCR, using the initially defined specific primers. The <strong>amplicons<\/strong> obtained are then sequenced by <strong>NGS<\/strong>, following the addition of sequencing adaptors. <strong>Multiplexing<\/strong> approaches enable us to sequence several <strong><a title=\"Amplicon : d&#xE9;finition\" href=\"https:\/\/fr.wikipedia.org\/wiki\/Amplicon\">amplicons<\/a><\/strong> from different samples in parallel.  <\/p>\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-04478499 wp-block-buttons-is-layout-flex\" style=\"margin-top:20px;margin-bottom:20px\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link has-elementor-28-fc-130-color has-elementor-primary-background-color has-text-color has-background has-link-color wp-element-button\" href=\"https:\/\/www.genoscreen.fr\/en\/genomic-services\/request-a-quote\/\" style=\"border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-left-radius:0px;border-bottom-right-radius:0px\">Request a quote<\/a><\/div>\n<\/div>\n\n<div class=\"wp-block-group has-elementor-28-fc-130-color has-elementor-secondary-background-color has-text-color has-background has-link-color wp-elements-5509eaee2b27e8f5a5b2ed6298782718 is-layout-flow wp-block-group-is-layout-flow\" style=\"padding-top:24px;padding-right:24px;padding-bottom:24px;padding-left:24px\">\n<h2 class=\"wp-block-heading panel-title has-elementor-28-fc-130-color has-text-color has-link-color wp-elements-a711efefc584eb12eafb80605ee16d43\">The Metabiote\u00ae solution<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Metabiote\u00ae<\/strong> is a dedicated, innovative, optimized, standardized solution developed by GenoScreen. It integrates targeted <strong><a href=\"https:\/\/www.genoscreen.fr\/en\/genoscreen-expertise\/the-analysis-of-microbial-communities\/\" type=\"page\" id=\"116\">metagenomic analysis of microbial communities<\/a><\/strong>. <\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>A library preparation methodology that limits between-sample bias.<\/li>\n\n\n\n<li>A diverse range of targets:<br\/>\u2022 <strong>bacterial<\/strong> targets: 16S rDNA<br\/>\u2022 <strong>fungal<\/strong> targets: 18S, ITS1 and\/or ITS2 rDNA<\/li>\n\n\n\n<li>A <a href=\"https:\/\/www.genoscreen.fr\/en\/genomic-services\/bioinformatics\/\" type=\"page\" id=\"112\">bioinformatics<\/a> pipeline that has been optimized and automated for <strong>high-precision<\/strong> analyses, easy comparisons and statistical analyses, and a high degree of responsiveness.<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-04478499 wp-block-buttons-is-layout-flex\" style=\"margin-top:20px;margin-bottom:20px\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link has-elementor-aa-90-b-4-d-color has-elementor-28-fc-130-background-color has-text-color has-background has-link-color wp-element-button\" href=\"https:\/\/www.genoscreen.fr\/en\/genoscreen-expertise\/bacterial-and-fungal-profiling\/\" style=\"border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-left-radius:0px;border-bottom-right-radius:0px\">Learn more<\/a><\/div>\n<\/div>\n<\/div>\n\n<h3 class=\"wp-block-heading\">Exome sequencing<\/h3>\n\n<p class=\"wp-block-paragraph\">This type of sequencing is essentially used to:<\/p>\n\n<ul class=\"wp-block-list\">\n<li>Screen for <strong>mutations in genes<\/strong> involved in regulatory pathways<\/li>\n\n\n\n<li>Screen for <strong>mutations<\/strong> (SNPs, indels, etc.) associated with a particular <strong><a title=\"S&#xE9;quen&#xE7;age cibl&#xE9; pour la sant&#xE9;\" href=\"https:\/\/www.genoscreen.fr\/en\/healthcare-nutrition\/\">disease<\/a><\/strong> (cancer, coagulopathies, etc.).<\/li>\n<\/ul>\n\n<p class=\"wp-block-paragraph\">We use the <a title=\"Genoscreen, partenaire de Roche\" href=\"https:\/\/diagnostics.roche.com\/content\/dam\/diagnostics\/Blueprint\/en\/rochesequence\/worldwide\/resources\/009__1000066_SeqCapEZtargetEnrich_Bro_VIEW.pdf\">NimbleGen SeqCap EZ protocol (Roche)<\/a> for exome sequencing.<\/p>\n\n<h3 class=\"wp-block-heading\">Personalized sequencing<\/h3>\n\n<p class=\"wp-block-paragraph\">After custom probe design, your regions of interest are enriched prior to sequencing which simplifies the subsequent data analysis. This protocol can be set up for any organism with an available reference genome. <\/p>\n\n<h2 class=\"wp-block-heading\">GenoScreen&#8217;s assets<\/h2>\n\n<ul class=\"wp-block-list\">\n<li>A dedicated team of scientists for each project.<\/li>\n\n\n\n<li>Comprehensive support with the elaboration of an experimental plan and project implementation.<\/li>\n\n\n\n<li>Assistance with data handling and interpretation of the results.<\/li>\n<\/ul>\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-04478499 wp-block-buttons-is-layout-flex\" style=\"margin-top:20px;margin-bottom:20px\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link has-elementor-28-fc-130-color has-elementor-primary-background-color has-text-color has-background has-link-color wp-element-button\" href=\"https:\/\/www.genoscreen.fr\/en\/genomic-services\/request-a-quote\/\" style=\"border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-left-radius:0px;border-bottom-right-radius:0px\">Request a quote<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Targeted sequencing allows specific analysis of targeted genes (or gene regions) on many samples in parallel. With NGS sequencing on MiSeq\u00ae, NextSeq500\u00ae or Hiseq\u00ae platforms, multiple genes can be evaluated with a smaller data volume, which is easier to manage and analyse than WGS datas. Applications Sequencing or targeted resequencing can achieve very specific objectives: [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":2290,"parent":177,"menu_order":11,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"page-categories":[158],"class_list":["post-182","page","type-page","status-publish","has-post-thumbnail","hentry","page-categories-genoscreen-services"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Targeted sequencing - An in-depth analysis of regions of interest - GenoScreen<\/title>\n<meta name=\"description\" content=\"Targeted sequencing enables the specific analysis of targeted genes or gene regions on a large number of samples in parallel.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.genoscreen.fr\/en\/genomic-services\/sequencing\/next-generation-sequencing-ngs\/targeted-sequencing\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Targeted sequencing - 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