Molecular barcoding (DNA barcoding) is a molecular taxonomy technique used to identify and genetically characterise a sample from a ubiquitous gene, generally belonging to the ribosomal, mitochondrial or chloroplast genome (COI, 16S, rbcL, etc.).
DNA barcoding applications
DNA barcoding can be used to:
- Identify species from an isolated sample
- Compare several samples from the same species or closely related species (phylogeny)
Identification by DNA barcoding
GenoScreen offers a complete molecular barcoding service and can handle your samples from DNA extraction onwards.
- Identification of isolated micro-organisms
- Identification of animal or plant species
For complex inital matrices (e.g. assemblies of bacterial populations), GenoScreen develops metabarcoding solutions using NGS amplicon sequencing to obtain screenings of samples: soil fauna, intestinal flora, coral reefs, etc.
GenoScreen offers you a straight-forward service for identifying your micro-organisms by DNA sequencing and comparison with international databases (NCBI or other).
For species identification, the ribosomal DNA regions are adapted to your micro-organism. In addition, other genomic targets (rpoB, gyrB, panC, etc.) can be used to refine this identification and adapt it to your requirements (for example, discrimination within a group of species sharing the same ribosomal DNA sequence).
Identification services
bacteria
fungi
yeast
microalgae
Workflow for isolated microorganisms
Extraction of your samples
from isolated colonies
PCR amplification on the appropriate genomic target
16S, 18S, 28S, ITS, ...
Forward/reverse
sequencing
Cleaning and assembly
in consensus, then comparison
Assignment
Our offer
Performed on :
- Agar (Petri dishes)
- Liquid culture plate
- FTA card or other matrix
From extracted DNA or thermolysate. We will compare your cleaned and assembled sequences with the international NCBI database.
Once the assignment has been completed, you will receive :
- Raw sequences and chromatograms (.seq/.ab1)
- Sequence consesus (.txt/.FA)
- Analysis report with homology percentages and assignment per sample
Additional solutions
In addition to identification, we are also able to produce phylogenetic trees according to your needs (comparison of samples with each other or within a panel of reference sequences).
Depending on your needs, we can also offer you other targets that are more specific to a particular genus, particularly for species that are too close genetically to the default target (for example, the panC target for the Bacillus genus).
GenoScreen also offers :
for strains whose species is known and for which a typing scheme is available
16S, 18S, 28S, ITS, ...
for more precise characterisation of your micro-organisms
