Metagenomics allows you to understand a complex environment and to characterize the species that are present (from bacterial, fungal, viral or eukaryotic origin). This technological approach does not require preparation and / or pre-insulation of complex environments exept the DNA extraction. Metagenomics can be performed on any type of samples, water, soil, tissue, feces, sputum, food, ..
Our team has an extensive experience in metagenomics and can assist you whatever the nature of your original sample. Metagenomic analysis is commonly done by amplifying a region of a ribosomal gene (eg ADNr16S, ADNr18S, 23S, 18S, ITS2 region) or a specific gene (CytB, nosZ, hsp65 etc...) or combination of several targets. The sequencing is mainly done on GsFLX sequencer due to the length of these reads.
For whole metagenomics approach we offer a blend of HiSeq2000 and GsFLX .
Benefit from our experience and trainings in Bioinformatics to sustain your metagenomics projects.
Genoscreen has an access to a high security laboratory (P3).
Metagenomics on all kind of samples:
- environment (water, lake and marine, environments, soil, sediment, mud...)
- food products
Sébastien Monchy, Giovanna Sanciu, Marlène Jobard, Serena Rasconi, Mélanie Gerphagnon, Magali Chabé, Amandine Cian, Dionigia Meloni, Nathalie Niquil, Urania Christaki, Eric Viscogliosi and Télesphore Sime-Ngando
Exploring and quantifying fungal diversity in freshwater lake ecosystems using rDNA cloning/sequencing and SSU tag pyrosequencing
Environmental Microbiology (2011)