Molecular barcoding (also known as DNA barcoding) is a molecular taxonomy technique for genetically characterizing a sample referring to a gene from the mitochondrial genome (COI, 16S, etc.).

Applications

This technique can be used to classify individuals from unknown species or to characterize a sample by comparison with a database.

Barcoding is notably used to:

  • Analyze samples.
  • Identify plant or animal species (taxonomy).
  • Analyze and compare levels of biodiversity in complex samples (metabarcoding).

GenoScreen - Services provided

GenoScreen offers a comprehensive molecular barcoding service:

  • Extraction from a large range of matrices stored in ethanol, propylene, glycol, etc.
  • Amplification of an appropriate region of the mitochondrial or chloroplast genome (COI, 16S, Cytb, rbcL, matk, etc.), depending on the species being studied.
  • Sequencing.
  • Sequence assembly and alignment on a reference sequence or the NCBI database.

For pure and unique samples, GenoScreen also offers Sanger sequencing services.

If your initial matrix is complex (e.g. an assembly of bacterial populations from the soil, intestinal flora, coral reefs, etc.), GenoScreen can develop metabarcoding solutions via NGS amplicon sequencing.

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