Sequencing
Next generation and Sanger sequencing
With more than 10 years experience, Genoscreen proposes a complete range of sequencing services that meet the requirement of nowadays genetic and genomic research projects.
Flexibility, hotline, direct relation with our staff: Genoscreen guarantees a high quality service.
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Our laboratory is equipped with the most efficient sequencing technologies: ABI 3730XL, 454 GS-FLX Roche Diagnostics. We also propose Illumina HiSeq 2000 services.
From classic Sanger DNA sequencing in tubes and 96 well plates, up to next generation sequencing applications such as metagenomics, genomes sequencing or mutation detection, find out about all of our DNA sequencing services :
> Sanger sequencing (3730XL®, Applied Biosystems®)
Sanger sequencing for PCR products, plasmidic DNA, BACs, cosmids...
> Genomes sequencing and resequencing
Genomes sequencing is proposed for all kind of organisms (bacteria, parasites, mold, yeasts, fungi, virus, plants...)
> CHIP-Seq
ChipSeq sequencing aims to isolate regions of a genome that are involved in gene regulation. They contain consensus sequences on which transcription factor acts to favor or inhibit the expression of a gene.
Knowing where these consensus sites are located, and which genes they are associated with gives information regarding the site of action of the transcription factor in the genome.> Mutations detection
According to your project, we propose low throughput services (primer design, PCR and sequencing) and high throughput solutions with enrichment, sequence capture and next generation sequencing using Roche® and Illumina® technologies.
We propose a pipeline of analyses dedicated to mutations identification and selection of SNPs of interest. For confirmation and biological validation of identified SNPs, we propose a complementary analysis on ABI ® 3730XL capillary sequencer.
> Micro-organisms identification
Genoscreen proposes several solutions for micro-organisms identification.
