MIRU-VNTR typing services and kits
MIRU-VNTR typing of mycobacteria (9, 12, 15 or 24 loci)
For an extensive analyse of your mycobacteria (M. tuberculosis, M. bovis…), Genoscreen proposes strains typing by the internationally standardized MIRU-VNTR method (mycobacterial interspersed repetitive unit -variable number tandem repeat).
It is based on multi-locus analysis of tandem repeat sequences. The MIRU-VNTR markers also enables to look for the strains genetic origin. We can work on your loci combination or suggest you one.
If you wish to outsource your strains typing, there is nothing easier: send us your DNAs or your inactivated strains, we will perform the typing, and you will receive the MIRU code of your samples within a few days.
You can send us inactivated cultures, lysates (50 uL) or DNA extracts (5 uL at 100 ng/uL).
We also propose complementary analysis:
- LSP analysis
- Resistance : atpE, pncA, rpob, katG, inhA analysis
- Speciation of atypic mycobacteria (hsp65)
- SNP genotyping
MIRU-VNTR Typing Kit - Mycobacterium tuberculosis complex 24 loci - 100 tests (50 tests version and custom triplex kits available on request)
Our MIRU-VNTR typing kits represent a complete package to facilitate implementation and optimal use of MIRU-VNTR typing on Applied Biosystems® sequencers, directly in research and hospital laboratories. Our scientists also can train you in your own laboratory. Data manager software is also available.
Applications:
- Research: strain population structure, evolution, comparative strain virulence properties
- Public health: TB control and surveillance
- Clinical trials: distinction between true relapse and exogenous infection in case of treatment failures
- Clinical management: detection of laboratory cross-contamination, clonal complexity
On-site MIRU-VNTR training is also proposed . It includes the set up of all MIRU-VNTR technique stages in user's laboratory, and users' technical and scientific training.
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Complementary products:
- Calibration kit : Preliminary step for use of the MIRU-VNTR typing kit, it enables MIRU-VNTR method implementation and validation in user's lab.
- MIRU-VNTR Data Manager: software tool for Genemapper® exported data management
- Validation kit: for regular quality controls
