Micro-organisms typing
MLST-MLSA-MLVA microorganisms typing, adaptation of typing methods
Microorganisms typing by the MLST/MLSA/MLVA method (bacterias and other microorganisms)
Reference laboratories and centers, control and surveillance organisms, hospitals, epidemiologists, manufacturers, Genoscreen proposes you the genotyping your micro-organisms from DNA extracts, lysates or isolated strains on agar by the MLST, MLSA or MLVA method.
MLST works for most bacteria, as well as for some fungi and yeasts. MLSA is used when housekeeping genes are not polymorphic enough, so we use both housekeeping and virulence genes. MLVA focuses on variable number of tandem repeats (VNTR) instead of on the sequence of the micro-organism. It uses fragment analysis. Repeated sequences regions and primers are different depending on the species.
We can also make the submission of the new alleles and profiles found in the reference library and send you the username and password.
No standardized typing method for your micro-organism? A project based on a specific number of loci? We can set up the typing technique of your strains and perform it, from:
- an adapted typing method
- available published conditions
- a reference genome
- after the studied genome whole sequencing
Some developed and available typing schemes:
- MLVA Francisella tularensis
- MLVA and MLST Escherichia coli
- MLSA Mycobacterium intracellulare
- MLST Bacillus of the cereus group
- MLST Staphylococcus hominis
- MLST Streptococcus pneumoniae
- MLST Acinetobacter baumanii
Any MLST scheme from pubmlst.org and mlst.net databases can be quickly set up on request.
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Staphylococcus aureus typing by the SPA method
For the analysis of your Staphylococcus aureus strains, Genoscreen offers typing by sequencing of the gene encoding protein A, from DNA extracts or isolated strains on agar.
MIRU-VNTR typing (9, 12, 15 or 24 loci)
> Visit our MIRU-VNTR typing dedicated page
You might also like to visit the bacterial genomes sequencing et metagenomics pages
