Geno Sat microsatellites libraries and genotyping
Production of microsatellites libraries by next generation sequencing
A standardized method
Based on high throughput sequencing, the technique* is validated on 160 different libraries, sequenced by Genoscreen.
An unequalled efficiency
From 1 μg of genomic DNA, this technology produces a minimum of 8 000 sequences in a record time of 2 months.
A flexible offer
To fit your objectives, Genoscreen proposes a simple and attractive solution: Geno Sat for the library production, Geno Sat Pack that also includes the polymorphic markers validation, and Geno Sat Pack Plus that integrates the genotyping based on the validated markers.
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Geno Sat
Genoscreen proposes a solution of microsatellites libraries creation:
- Production of microsatellites markers by cloning and capillary sequencing (3730XL®, Applied Biosystems®) and by high throughput pyrosequencing (GS FLX®, Roche Diagnostics®)
- Development and validation of microsatellites markers by fragment analysis on capillary sequencer (3730XL®, Applied Biosystems®)
- Genotyping of microsatellites markers by fragment analysis on capillary sequencer (3730XL®, Applied Biosystems®)
We propose the definition of the optimal PCR conditions for microsatellites analysis, the performing of multiplexed PRC, and the genotyping of the samples on capillary sequencer. We can also perform the associated analysis.
- Provision of size marker
- Integration of quality controls
- Storage of stock samples during 4 weeks
- Available combinations of fluorophores : FAM-VIC/HEX-PET-NED or FAM-HEX-NED-ROX
- Measures guaranteeing the confidentiality of the successful information and the obtained results
Geno Sat Pack
Geno Sat + Biologic validation of primers pairs and identification of
polymorphic microsatellites markers using samples representing the
intra-specific genetic variability.
Geno Sat Pack Plus
Geno Sat Pack +Genotyping on a large population of samples, using
the validated markers.
* Proccess: GENOSCREEN – INRA – Montpellier SUPAGRO– Provence university.
Genotyping
Genoscreen proposes various technologies and applications :
Microsatellites markers
SNPs markers
Specific application to transgenic animals genotyping
Specific applications to molecular microbiology
Microsatellites markers
Genoscreen proposes a solution of microsatellites library creation:
1/ Production of microsatellites markers by cloning and capillary sequencing (3730XL®, Applied Biosystems®) and by high throughput pyrosequencing (GS FLX®, Roche Diagnostics®)
2/ Development and validation of microsatellites markers by fragment analysis on capillary sequencer (3730XL®, Applied Biosystems®)
3/ Genotyping of microsatellites markers by fragment analysis on capillary sequencer (3730XL®, Applied Biosystems®)
We propose the definition of the optimal PCR conditions for microsatellites analysis, the performing of multiplexed PRC, and the genotyping of the samples on capillary sequencer. We can also perform the associated analysis.
- Provision of size marker
- Integration of quality controls
- Storage of stock samples during 4 weeks
- Available combinations of fluorophores : FAM-VIC/HEX-PET-NED or FAM-HEX-NED-ROX
- Measures guaranteeing the confidentiality of the successful information and the obtained results
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SNPs markers
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Genoscreen helps you to find the solution that is the most adapted to your project :
- Genotyping of SNPs markers by the TaqMan® technology
(recommended for the analysis of less than 10 SNPs)
- Genotyping of SNPs markers by the Sequenom® technology
(recommended for the analysis of 10 to 47 SNPs)
- Genotyping of SNPs markers by the Veracode® technology
(recommended for the analysis of 48 to 1* or 3* 384 SNPs)
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Specific application to transgenic animals genotyping
By specific amplification profile, we propose to validate the genotype of your transgenic animals.
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Specific applications to molecular microbiology
- Mycobacterium tuberculosis typing by the MIRU method (9, 12, 15 or 24 MIRU markers)
For the analysis of your Mycobacterium tuberculosis strains, Genoscreen offers typing from DNA extract or isolated strains on agar by the MIRU-VNTR method. This internationally standardized method is based on the multi-locus analysis of the number of tandem repeats, so for each samples, you will receive the associated MIRU code. The MIRU-VNTR markers also allow you to search for genetic strains.
- MIRU-VNTR Typing Kit -Mycobacterium tuberculosis complex 24 loci - 100 tests
Our MIRU-VNTR typing kits represent an innovative and complete package designed for facilitating implementation and optimal use of MIRU-VNTR typing on Applied Biosystems ® sequencers, directly in clinic or research labs
A multi-task software for data management, as well as on-site training are also available.
Applications :
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- Research: strain population structure, evolution, comparative strain virulence properties
- Public health: TB control and surveillance
- Clinical trials: distinction between true relapse and exogenous infection in case of treatment failures
- Clinical management: detection of laboratory cross-contamination, clonal complexity
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- Complementary products:
The calibration kit is designed for implementation and validation of MIRU-VNTR technique in user's laboratory, including Genemapper and run parameters modules, allelic ladders to define bins and validation samples.
The validation kit is made of 12 samples with a known MIRU code and a validated DNA concentration. It enables to perform regular quality controls.
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